Functional redundancy of genes for sulphate activation enzymes in Rhizobium sp. BR816.

Microbiology
T LaeremansE Martínez-Romero

Abstract

The broad-host-range, heat-tolerant Rhizobium strain BR816 produces sulphated Nod metabolites. Two ORFs highly homologous to the Sinorhizobium meliloti nodPQ genes were isolated and sequenced. It was found that Rhizobium sp. BR816 contained two copies of these genes; one copy was localized on the symbiotic plasmid, the other on the megaplasmid. Both nodP genes were interrupted by insertion of antibiotic resistance cassettes, thus constructing a double nodP1P2 mutant strain. However, no detectable differences in Nod factor TLC profile from this mutant were observed as compared to the wild-type strain. Additionally, plant inoculation experiments did not reveal differences between the mutant strain and the wild-type. It is proposed that a third, functionally homologous locus complements mutations in the Nod factor sulphation genes. Southern blot analysis suggested that this locus contains genes necessary for the sulphation of amino acids.

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Citations

May 3, 2001·Molecular Plant-microbe Interactions : MPMI·C SnoeckJ C Promé
Oct 13, 1998·Molecular Plant-microbe Interactions : MPMI·J L Folch-MallolM Megías
Sep 24, 1999·Molecular Plant-microbe Interactions : MPMI·T LaeremansJ Vanderleyden
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·I M López-Lara, O Geiger
Apr 19, 2012·Environmental Microbiology·Mark Dopson, D Barrie Johnson
Jan 20, 2007·FEMS Microbiology Letters·Roseline RemansJos Vanderleyden
Mar 10, 2004·Applied and Environmental Microbiology·Martine MorisJan Michiels

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