Functional redundancy of the human CCL4 and CCL4L1 chemokine genes

Biochemical and Biophysical Research Communications
O M Zack HowardWilliam S Modi

Abstract

CCL4 and CCL4L1 are two CC chemokine genes located at chromosome 17q21 whose mature proteins differ at only a single amino acid. Abundant functional information exists for CCL4, however, CCL4L1 has only recently been recognized as a distinct gene, thus information describing it is wanting. The CCL4L1 protein was synthesized in Escherichia coli and compared with the CCL4 protein. Competitive binding studies using HEK-293/CCR5 cells produced comparable EC50 values for the two proteins. Similarly, chemotaxis assays with cells expressing CCR1, CCR3, or CCR5 revealed no substantial differences. CCL4L1 was somewhat more effective at inhibiting HIV-1 replication in PBMCs than was CCL4, however the difference was not statistically significant. These data combined with the observation of individual variation in CCL4L1 gene copy number [Eur. J. Immunol. 32 (2002) 3016, Genomics 83 (2004) 735] support the contention that the CCL4 and CCL4L1 proteins have redundant functions.

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Citations

May 27, 2011·The Journal of Investigative Dermatology·Edurne PedrosaJosé-Manuel Carrascosa
Jul 15, 2005·Annals of the New York Academy of Sciences·David NaorEva A Turley
Apr 15, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Avani BharuthramCaroline T Tiemessen
Nov 26, 2010·European Journal of Pharmacology·Kristina LundbergMalin Lindstedt
Mar 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Malavika S GiriLuis J Montaner
Dec 2, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tasha BarrIlhem Messaoudi
May 13, 2020·Parasite Immunology·Gillian CoakleyNicola Laraine Harris

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