Functional redundancy of two Pax-like proteins in transcriptional activation of cyst wall protein genes in Giardia lamblia.

PloS One
Shen-Fung ChuangChin-Hung Sun

Abstract

The protozoan Giardia lamblia differentiates from a pathogenic trophozoite into an infectious cyst to survive outside of the host. During encystation, genes encoding cyst wall proteins (CWPs) are coordinately induced. Pax family transcription factors are involved in a variety of developmental processes in animals. Nine Pax proteins have been found to play an important role in tissue and organ development in humans. To understand the progression from primitive to more complex eukaryotic cells, we tried to identify putative pax genes in the G. lamblia genome and found two genes, pax1 and pax2, with limited similarity. We found that Pax1 may transactivate the encystation-induced cwp genes and interact with AT-rich initiatior elements that are essential for promoter activity and transcription start site selection. In this study, we further characterized Pax2 and found that, like Pax1, Pax2 was present in Giardia nuclei and it may specifically bind to the AT-rich initiator elements of the encystation-induced cwp1-3 and myb2 genes. Interestingly, overexpression of Pax2 increased the cwp1-3 and myb2 gene expression and cyst formation. Deletion of the C-terminal paired domain or mutation of the basic amino acids of the paired domain re...Continue Reading

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Citations

Jul 25, 2014·PLoS Neglected Tropical Diseases·María C MerinoAndrea S Rópolo
Sep 1, 2015·Seminars in Cell & Developmental Biology·Vanessa R Paixão-CôrtesMaria Cátira Bortolini
Apr 23, 2014·Biochimica Et Biophysica Acta·Cecilia V VranychAndrea S Rópolo
Dec 15, 2011·The Journal of Biological Chemistry·Chao-Cheng ChoChin-Hung Sun
Feb 14, 2021·Biochimica Et Biophysica Acta. General Subjects·Jui-Hsuan WuChin-Hung Sun

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Datasets Mentioned

BETA
GSE30875

Methods Mentioned

BETA
PCR
electrophoresis
immunoprecipitation
ChIP
in vitro transcription
Feature Extraction
electrophoretic mobility shift
Co-immunoprecipitation

Software Mentioned

ImageQuant
ClustalW
Feature Extraction
BLAST

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