Functional relationships of human T-lymphocytes and interleukins: I. Different accessory cell requirements of human T-lymphocyte subpopulations for generation of interleukin-2

Immunobiology
A J Ulmer, H D Flad

Abstract

Interleukin-2 (Il-2) producing human T-lymphocyte subsets were isolated by discontinuous density gradient centrifugation and their requirements for helper monocytes were investigated. The results show that T-lymphocytes from fractions 2, 3, and 4 from the density gradient (high density lymphocytes) which contain up to 75% Fcmu receptor-bearing cells, and less than 0.2% monocytes could be stimulated by PHA for the production of Il-2 only in the presence of additional monocytes. T-lymphocytes from fractions 6 and 7 and in most experiments also from fraction 5 (low-density lymphocytes containing between 20% and 50% Fcmu receptor bearing cells, but still 0.2% up to 30% monocytes) could be stimulated by PHA for the production of Il-2 also without additional monocytes. Dose-response experiments with various numbers of monocytes added to the system showed the following: the higher the density of a cell population, the higher the number of monocytes required for help in the stimulation of Il-2 production. However, the production of Il-2 by high-density lymphocytes did not reach the Il-2 production by low-density T-lymphocytes even in the presence of high numbers of additional monocytes. Dose-response experiments indicated that the diff...Continue Reading

References

Jul 19, 1979·Nature·E L Larsson, A Coutinho
Apr 1, 1980·The Journal of Experimental Medicine·E L ReinherzS F Schlossman
Feb 1, 1980·European Journal of Immunology·E L Larsson, A Coutinho

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Citations

Jul 1, 1989·Molecular Immunology·S UmlandC Martens
Oct 1, 1984·Australian and New Zealand Journal of Medicine·G McLennan, N DeYoung

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