Functional relationships of the sialyltransferases involved in expression of the polysialic acid capsules of Escherichia coli K1 and K92 and Neisseria meningitidis groups B or C

The Journal of Biological Chemistry
S Steenbergen, Eric R Vimr

Abstract

Polysialic acid (PSA) capsules are cell-associated homopolymers of alpha2,8-, alpha2,9-, or alternating alpha2,8/2,9-linked sialic acid residues that function as essential virulence factors in neuroinvasive diseases caused by certain strains of Escherichia coli and Neisseria meningitidis. PSA chains structurally identical to the bacterial alpha2,8-linked capsular polysaccharides are also synthesized by the mammalian central nervous system, where they regulate neuronal function in association with the neural cell adhesion molecule (NCAM). Despite the structural identity between bacterial and NCAM PSAs, the respective polysialyltransferases (polySTs) responsible for polymerizing sialyl residues from donor CMP-sialic acid are not homologous glycosyltransferases. To better define the mechanism of capsule biosynthesis, we established the functional interchangeability of bacterial polySTs by complementation of a polymerase-deficient E. coli K1 mutant with the polyST genes from groups B or C N. meningitidis and the control E. coli K92 polymerase gene. The biochemical and immunochemical results demonstrated that linkage specificity is dictated solely by the source of the polymerase structural gene. To determine the molecular basis for ...Continue Reading

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Citations

Mar 30, 2010·Applied Microbiology and Biotechnology·M A Ferrero, Leandro Rodríguez Aparicio
Apr 25, 2012·Applied Microbiology and Biotechnology·Yanhong Li, Xi Chen
Feb 26, 2005·Infection and Immunity·S SteenbergenEric R Vimr
Aug 21, 2012·Journal of Bacteriology·Lavanya RishishwarI King Jordan
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Eric R VimrS Steenbergen
Jun 8, 2006·Annual Review of Biochemistry·Chris Whitfield
Apr 15, 2014·Nature Chemical Biology·Timothy G KeysR Gerardy-Schahn
May 9, 2006·Molecular Microbiology·Eric R Vimr, S Steenbergen
Sep 7, 2013·Chembiochem : a European Journal of Chemical Biology·Friedrich FreibergerMark von Itzstein
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Hanne L P Tytgat, Sarah Lebeer

Related Concepts

Polysialic acid
Carbohydrate Sequence
Alkalescens-Dispar Group
Episomes
Polysaccharides, Bacterial
Sialic Acids
Ectosialyltransferase
Mutagenesis, Site-Directed
Determination, Sequence Homology
Bacterial Capsules

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