PMID: 7426435Jul 1, 1980

Functional restoration of ACD-blood by pyrodoxal 5'-phosphate

British Journal of Haematology
N MaedaT Shiga


Successful application of pyridoxal 5'-phosphate (PLP) to restore the oxygen transport function of ACD-stored blood is described. PLP is readily incorporated into ACD-erythrocytes by both carrier-mediated transport (in which ATP may participate) and passive diffusion. Plasma proteins (up to 2.5%) and inorganic phosphate (up to 40 mM) do not affect the incorporation of PLP, though more than 25 mM inorganic phosphate is necessary for the maintenance of ATP levels. Increasing the PLP concentration and/or decreasing the packed cell volume in the medium, increases the incorporation of PLP. Incubation of erythrocytes with PLP at pH 7.0 is most suitable for incorporation of PLP and the maintenance of ATP levels. PLP incorporated into erythrocytes restores the oxygen transport function of the ACD-erythrocytes, though decreased haem--haem interaction is observed. A procedure for the clinical application of PLP-loaded erythrocytes is suggested.


Nov 1, 1976·British Journal of Haematology·N MaedaT Shiga
Dec 1, 1976·FEBS Letters·R Flückiger, K H Winterhalter
Dec 1, 1976·Biochemical Medicine·L R Solomon, R S Hillman
Sep 1, 1978·Respiration Physiology·M Samaja, L Gattinoni
Jul 1, 1975·Analytical Biochemistry·C J Chern, E Beutler
Oct 5, 1977·Journal of Molecular Biology·A ArnoneR Benesch
Jun 1, 1976·The Journal of Clinical Investigation·H F BunnP M Gallop
Apr 25, 1975·Annals of the New York Academy of Sciences·J D Hines
Nov 3, 1975·Biochemical and Biophysical Research Communications·H F BunnP M Gallop
Jan 1, 1972·Archives of Biochemistry and Biophysics·S K Srivastava, E Beutler
Aug 9, 1974·Nature·B B AndersonR Green
Jan 1, 1972·Cold Spring Harbor Symposia on Quantitative Biology·M F Perutz, L F TenEyck
May 28, 1973·Biochimica Et Biophysica Acta·S K Srivastava, E Beutler
Jan 1, 1973·Biology of the Neonate·E BursauxC F Poyart
Sep 12, 1972·Biochemistry·R E BeneschN Maeda
Jun 3, 1971·The New England Journal of Medicine·N MaedaR E Benesch
Sep 1, 1970·The Journal of Vitaminology·R Suzue, M Tachibana
Sep 1, 1971·The Journal of Clinical Investigation·B B AndersonC J Bateman
Mar 11, 1968·Biochimica Et Biophysica Acta·G R Bartlett
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·O AkerblomC Högman
Dec 10, 1968·The Journal of Vitaminology·K Yamada, M Tsuji
Feb 1, 1969·The Journal of Clinical Investigation·H F BunnC E Shields
Jul 1, 1967·Archives of Biochemistry and Biophysics·A Chanutin, R R Curnish
Apr 1, 1967·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Hamfelt
Jan 1, 1967·Annual Review of Biochemistry·P Fasella

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Blood Preservation
Serum Proteins
Hematocrit Procedure
Hydrogen-Ion Concentration
Pyridoxal Phosphate

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.