PMID: 7426435Jul 1, 1980

Functional restoration of ACD-blood by pyrodoxal 5'-phosphate

British Journal of Haematology
N MaedaT Shiga

Abstract

Successful application of pyridoxal 5'-phosphate (PLP) to restore the oxygen transport function of ACD-stored blood is described. PLP is readily incorporated into ACD-erythrocytes by both carrier-mediated transport (in which ATP may participate) and passive diffusion. Plasma proteins (up to 2.5%) and inorganic phosphate (up to 40 mM) do not affect the incorporation of PLP, though more than 25 mM inorganic phosphate is necessary for the maintenance of ATP levels. Increasing the PLP concentration and/or decreasing the packed cell volume in the medium, increases the incorporation of PLP. Incubation of erythrocytes with PLP at pH 7.0 is most suitable for incorporation of PLP and the maintenance of ATP levels. PLP incorporated into erythrocytes restores the oxygen transport function of the ACD-erythrocytes, though decreased haem--haem interaction is observed. A procedure for the clinical application of PLP-loaded erythrocytes is suggested.

References

Nov 1, 1976·British Journal of Haematology·N MaedaT Shiga
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Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Blood Preservation
Serum Proteins
Erythrocytes
Hematocrit Procedure
Hydrogen-Ion Concentration
Dioxygen
Orthophosphate
Pyridoxal Phosphate

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