PMID: 9425107Feb 28, 1998Paper

Functional role for the angiotensin II receptor (AT1A) 3'-untranslated region in determining cellular responses to agonist: evidence for recognition by RNA binding proteins

The Biochemical Journal
T J ThekkumkaraK M Baker

Abstract

We demonstrate a functional role for the 3'-untranslated region (3'-UTR) of the angiotensin II (Ang II) receptor subtype AT1A mRNA in Chinese hamster ovary (CHO-K1) cells by stably transfecting the coding region of the receptor gene with or without the 845 bp 3'-UTR. Two cell lines expressing similar levels of cell-surface receptors (with 3'-UTR, Bmax=571 fmol/mg protein; without 3'-UTR, Bmax=663 fmol/mg protein) were used in the present study. Both cell lines expressed high-affinity receptors (with 3'-UTR, Kd=0.83 nM; without 3'-UTR, Kd=0.82 nM), and binding studies with 125I-labelled Ang II in the presence of GTP[S] demonstrated that both coupled to heterotrimeric G-proteins. Despite these similarities, significant differences were observed for receptor-mediated cell signalling pathways. In cells without the 3'-UTR, Ang II stimulated an increase in cAMP accumulation (11-fold above control) and in cells with the 3'-UTR no stimulation was observed, which was consistent with previous observations in most endogenous Ang II receptor (AT1)-expressing cells. Activation of cAMP by Ang II in cells without the 3'-UTR correlated with an inhibition of DNA synthesis, determined by [3H]thymidine incorporation. Ang II-mediated responses wer...Continue Reading

Citations

Jun 13, 2006·Cell Calcium·Christine M MisquittaAshok K Grover
Apr 6, 2005·Biochemical and Biophysical Research Communications·Anja BergerGeorg Nickenig
Nov 27, 2010·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Adriana P Mendizábal-RuizMaria C Moran Moguel
Feb 1, 2008·Journal of the American Society of Nephrology : JASN·Zhong-Gao XuRama Natarajan
May 25, 2012·Middle East African Journal of Ophthalmology·Swetha S PhilipAndrew D Braganza
Jan 31, 2003·Molecular and Cellular Biology·Stéphane PelletierSylvain Meloche
Feb 11, 2012·Molecular Endocrinology·Rekha YesudasThomas Thekkumkara
Apr 9, 2002·European Journal of Pharmacology·Lucimar P FrançaAntonio C M Paiva
Dec 22, 1999·Biochemical and Biophysical Research Communications·A PendeG Lotti
Sep 25, 2002·Life Sciences·Arpad PalfiKaroly Gulya
Feb 4, 1999·Regulatory Peptides·W G Thomas
Apr 18, 2020·Molecular Therapy. Methods & Clinical Development·Nishat SultanaLior Zangi

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