PMID: 6113843May 26, 1981Paper

Functional role of acidic ribosomal proteins. Interchangeability of proteins from bacterial and eukaryotic cells

Biochemistry
F Sánchez-MadridJuan P G Ballesta

Abstract

Core particles derived from yeast ribosomes by treatment with 50% ethanol and 0.4 M NH4Cl (P0.4 cores) are derived of the acidic proteins L44/45 functionally equivalent to the bacterial proteins L7 and L12. These bacterial proteins are able to reconstitute the EF-2-dependent GDP binding capacity of the yeast cores but not their GTPase activity. On the other hand, yeast particles prepared in similar conditions but in the presence of 1 M NH4Cl (P1.0 cores) lose proteins L44/45, L15, and S31. These particles are able to reconstitute both activities by the bacterial proteins L7 and L12. Proteins L15 and S31 somehow affect the interaction of bacterial proteins L7 and L12 with the yeast particles. Indeed, in their presence only one dimer of L7 and L12 is bound to the P0.4 cores, while in their absence (P1.0 cores) the amount of bacterial proteins retained by the yeast particles is doubled. Elongation factor EF-2 seems to play an important role in the binding of the bacterial proteins to the yeast cores. Our results suggest that the two dimers of L7 and L12 normally present in the ribosomes might play a different functional role, one of the dimers being related to the binding of the substrate and the other one involved in the GTPase a...Continue Reading

References

Aug 1, 1979·European Journal of Biochemistry·F Sánchez-MadridJ P Ballesta
Mar 15, 1979·Biochemical and Biophysical Research Communications·F Sánchez-MadridJ P Ballesta
Jan 1, 1979·Methods in Enzymology·W M Kemper, W C Merrick
May 1, 1976·Nucleic Acids Research·J P BallestaD Vázquez
Feb 15, 1977·European Journal of Biochemistry·R ReyesJ P Ballesta
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W MöllerD Richter
Oct 15, 1975·FEBS Letters·T Kruiswijk, R J Planta
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G StöfflerK H Rak
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans

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Citations

Apr 1, 1994·Journal of Molecular Evolution·D Liao, P P Dennis
Jan 1, 1982·Molecular & General Genetics : MGG·L M SabatiniW Y Chooi
Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
Aug 27, 1990·Biochimica Et Biophysica Acta·M T Saenz-RoblesJ P Ballesta
Oct 1, 1993·Parasitology Today·M J LevinA G Schijman
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K ElkonN Brot
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T UchiumiR R Traut
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T Naranda, J P Ballesta
Sep 25, 1991·Nucleic Acids Research·Y L Chan, I G Wool
Nov 15, 1988·European Journal of Biochemistry·M T Sáenz-RoblesJ P Ballesta
Nov 27, 1981·Biochimica Et Biophysica Acta·F J VidalesJ P Ballesta
Jan 17, 2012·Vaccine·Alina Rodríguez-MallonMario Pablo Estrada
May 12, 2015·Ticks and Tick-borne Diseases·Alina Rodríguez-MallonMario Pablo Estrada
May 18, 1999·Archives of Biochemistry and Biophysics·I KusserA T Matheson
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·I V MitroshinA G Gabdulkhakov
May 1, 1990·Molecular and Cellular Biology·M RemachaJ P Ballesta

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