Functional role of alternative splicing in pituitary P2X2 receptor-channel activation and desensitization

Molecular Endocrinology
Taka-aki KoshimizuStanko S Stojilkovic

Abstract

Although ATP-gated ion channel (P2XR) expression is high among anterior pituitary cells, identification of the receptor subtypes and their selective expression within subpopulations of cell types, as well as their physiological role(s), are incompletely characterized. In this study, we focused on the expression and activity of the P2X2R subtype in anterior pituitary cells. Our results indicate that the primary P2X2R gene transcript in pituitary cells undergoes extensive alternative splicing, with generation of six isoforms. Two of these isoforms encode functional channels when expressed in GT1 or HEK293 cells: the wild-type P2X2R and the spliced isoform P2X2-2R, which lacks a stretch of carboxyl-terminal amino acids (Val370-Gln438). Four other clones showed different alterations, including an interfered reading frame starting in the first transmembrane domain and a 27-amino acid deletion in the large extracellular loop. When expressed separately or in combination with wild-type channels, these clones were nonfunctional. In single cell Ca2+ current and cytosolic Ca2+ concentration ([Ca2+)i) measurements, the P2X2R and P2X2-2R had similar EC50 values for ATP and time courses for activation and recovery from desensitization but di...Continue Reading

Citations

May 2, 2012·Purinergic Signalling·Karina Kaczmarek-HájekAnnette Nicke
May 2, 2012·The Journal of General Physiology·Anmar KhadraStanko S Stojilkovic
Jul 9, 2011·Pharmacological Reviews·Claudio CoddouStanko S Stojilkovic
Dec 17, 2009·American Journal of Physiology. Endocrinology and Metabolism·Hana ZemkovaStanko S Stojilkovic
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
Jun 7, 2011·Reviews in the Neurosciences·Claudio CoddouJ Pablo Huidobro-Toro
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Jun 19, 2015·Cellular and Molecular Life Sciences : CMLS·David J HodsonNathalie C Guérineau
Sep 13, 2005·Annals of the New York Academy of Sciences·Stanko S StojilkovicHana Zemkova
Oct 12, 1999·The Journal of Physiology·F M SmithR D Murrell-Lagnado
Apr 12, 2018·International Journal of Molecular Sciences·Milos B RokicClaudio Coddou
May 2, 2006·Pflügers Archiv : European journal of physiology·Joel R GeverAnthony P D W Ford
May 9, 2009·Journal of Neurochemistry·Marie JindrichovaHana Zemkova
May 12, 2007·Journal of Neurophysiology·Krasimira Tsaneva-AtanasovaStanko S Stojilkovic
Jun 12, 2014·Pflügers Archiv : European journal of physiology·Hana ZemkovaStanko S Stojilkovic
Apr 25, 2003·American Journal of Physiology. Cell Physiology·Mu-Lan HeStanko S Stojilkovic
Feb 13, 2015·American Journal of Physiology. Cell Physiology·Claudio CoddouStanko S Stojilkovic
Sep 25, 2002·Physiological Reviews·R Alan North
Jun 27, 2017·Frontiers in Endocrinology·Stanko S StojilkovicHana Zemkova
Mar 29, 2001·Physiological Reviews·G Vassort
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Oct 4, 2002·The Journal of Biological Chemistry·Taka-aki KoshimizuGozoh Tsujimoto
Sep 13, 2003·The Journal of Biological Chemistry·Mu-Lan HeStanko S Stojilkovic
Dec 23, 1999·The Journal of Biological Chemistry·T KoshimizuS S Stojilkovic
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Jul 8, 2017·Molecular and Cellular Endocrinology·Hana Zemková, Stanko S Stojilkovic

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