PMID: 9557633Apr 29, 1998Paper

Functional role of hepatitis C virus chimeric glycoproteins in the infectivity of pseudotyped virus

Journal of Virology
L Martin LaggingR Ray

Abstract

The putative envelope glycoproteins of hepatitis C virus (HCV) likely play an important role in the initiation of viral infection. Available information suggests that the genomic regions encoding the putative envelope glycoproteins, when expressed as recombinant proteins in mammalian cells, largely accumulate in the endoplasmic reticulum. In this study, genomic regions which include the putative ectodomain of the E1 (amino acids 174 to 359) and E2 (amino acids 371 to 742) glycoproteins were appended to the transmembrane domain and cytoplasmic tail of vesicular stomatitis virus (VSV) G protein. This provided a membrane anchor signal and the VSV incorporation signal at the carboxy termini of the E1 and E2 glycoproteins. The chimeric gene constructs exhibited expression of the recombinant proteins on the cell surface in a transient expression assay. When infected with a temperature-sensitive VSV mutant (ts045) and grown at the nonpermissive temperature (40.5 degrees C), cells transiently expressing the E1 or E2 chimeric glycoprotein generated VSV/HCV pseudotyped virus. The resulting pseudotyped virus generated from E1 or E2 surprisingly exhibited the ability to infect mammalian cells and sera derived from chimpanzees immunized wit...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A J WeinerK A Crawford
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y K ShimizuH Yoshikura
Nov 30, 1992·Biochemical and Biophysical Research Communications·N KatoK Shimotohno
Mar 1, 1992·The Journal of Infectious Diseases·A M PrinceG Inchauspé
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M HijikataK Shimotohno
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·I SaitoY Ohta
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P ClaphamR A Weiss
Nov 1, 1982·The Journal of General Virology·J Závada
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·Q L ChooC Kuo
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·P FarciR H Purcell
Sep 1, 1994·Microbiological Reviews·J H Strauss, E G Strauss
Mar 1, 1994·Journal of Hepatology·E NissenE Schreier
Oct 1, 1994·Journal of Virology·H MizushimaK Shimotohno
Nov 1, 1993·Virology·R A Lamb
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y K ShimizuH Yoshikura
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller

❮ Previous
Next ❯

Citations

Mar 14, 2003·Journal of Virology·Stefan PöhlmannJane A McKeating
Feb 24, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alan Lee RothmanUNKNOWN HALT-C Trial Group
Apr 22, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marlène Dreux, Francois-Loïc Cosset
Sep 11, 2007·The FEBS Journal·Morgane RégeardJacques Le Seyec
Jul 8, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Budhaditya MazumdarRanjit Ray
Oct 12, 2012·International Journal of Hepatology·Garrick K Wilson, Zania Stamataki
Feb 19, 2013·Expert Review of Vaccines·Bertrand Bellier, David Klatzmann
Jul 16, 2004·Journal of Virology·Irene S LudwigTeunis B H Geijtenbeek
Dec 1, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jan Martin Berke, Darius Moradpour
Apr 16, 2005·The Journal of General Virology·Andrea HanikaGert Zimmer
Aug 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Valérie Castet, Darius Moradpour
May 25, 2007·Journal of Virology·Timothy L TellinghuisenCharles M Rice
Aug 5, 2011·Science Translational Medicine·Ranjit Ray
Mar 1, 2016·Virusdisease·Nilotpal Banerjee, Sumi Mukhopadhyay
Feb 19, 2004·Antiviral Chemistry & Chemotherapy·Kohji Moriishi, Yoshiharu Matsuura
Mar 5, 2003·The Journal of Experimental Medicine·Birke BartoschFrançois-Loïc Cosset
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann
May 9, 2000·Journal of Virology·S TakikawaT Miyamura
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Heidi BarthThomas F Baumert
Jun 24, 2003·Reviews in Medical Virology·Anne Op De Beeck, Jean Dubuisson
Dec 21, 2007·Journal of Virology·Keith MeyerRanjit Ray
Sep 15, 2005·Journal of Virology·Manujendra N SahaHiroo Hoshino
Mar 12, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anne Op De Beeck, Jean Dubuisson
Nov 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Birke BartoschRobert H Purcell
May 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mayla HsuJane A McKeating
Aug 13, 2003·The Journal of Biological Chemistry·Birke BartoschFrançois-Loïc Cosset
May 2, 2006·Archives of Virology·N JourdanS Bonatti
Jul 10, 1999·Journal of Virology·M FlintJ A McKeating

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.