Functional role of VDR in the activation of p27Kip1 by the VDR/Sp1 complex

Journal of Cellular Biochemistry
Hsuen-Tsen ChengWen-Chun Hung

Abstract

Our previous study demonstrate that vitamin D3 induces the binding of vitamin D3 receptor (VDR) to Sp1 transcription factor and stimulates p27Kip1 expression via the Sp1 consensus sequences in the promoter. Both VDR and Sp1 are transcriptional activators, it is unclear which protein functions as the transcription component of the VDR/Sp1 complex. To address this issue, we constructed the AF-2 deletion mutant of VDR and tested the effect of vitamin D3 on p27Kip1 expression. In consistent with our previous results, we found that expression of wild-type VDR in SW620 colon cancer cells, which expressed very low level of endogenous VDR, increased vitamin D3-stimulated p27Kip1 promoter activity and protein expression. On the contrary, expression of AF-2 deletion mutant had little effect. DNA affinity precipitation assay (DAPA) showed that both wild-type and deletion mutant of VDR bound to the DNA probe corresponding to the Sp1 binding site in the p27Kip1 promoter in a vitamin D3-dependent manner indicating deletion of AF-2 domain does not affect the interaction between VDR and Sp1. Chromatin immunoprecipitation (CHIP) assay also confirmed that VDR and its AF-2 deletion mutant bound to p27Kip1 promoter in vivo. We found that deletion ...Continue Reading

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Citations

Oct 20, 2012·World Journal of Gastroenterology : WJG·Khanh vinh quốc Lương, Lan Thi Hoàng Nguyễn
Jun 13, 2014·Oncology Reports·Magdalena MilczarekJoanna Wietrzyk
Sep 2, 2014·Computers in Biology and Medicine·Muhammad FaisalMalik Mumtaz Taqi
Oct 16, 2014·Journal of Molecular Endocrinology·Ana P IrazoquiClaudia G Buitrago
Nov 5, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Ana P IrazoquiClaudia G Buitrago
Mar 28, 2008·Biochemical and Biophysical Research Communications·Inken Wierstra
Apr 23, 2009·Journal of Cellular and Molecular Medicine·Darja ObradovicTheo Rein
Feb 9, 2017·Experimental Hematology·Huynh CaoChien-Shing Chen
Dec 29, 2011·American Journal of Alzheimer's Disease and Other Dementias·Khanh Vinh Quôc Lu'o'ng, Lan Thi Hoàng Nguyên
Jun 28, 2011·Expert Review of Respiratory Medicine·Nithya RamnathPaul J Christensen
Jul 8, 2018·The Journal of Steroid Biochemistry and Molecular Biology·Gemma Ferrer-MayorgaAlberto Muñoz

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