Functional roles of complexin in neurotransmitter release at ribbon synapses of mouse retinal bipolar neurons

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Thirumalini VaithianathanGary Matthews

Abstract

Ribbon synapses of photoreceptor cells and bipolar neurons in the retina signal graded changes in light intensity via sustained release of neurotransmitter. One molecular specialization of retinal ribbon synapses is the expression of complexin protein subtypes Cplx3 and Cplx4, whereas conventional synapses express Cplx1 and Cplx2. Because complexins bind to the molecular machinery for synaptic vesicle fusion (the SNARE complex) and modulate transmitter release at conventional synapses, we examined the roles of ribbon-specific complexin in regulating release at ribbon synapses of ON bipolar neurons from mouse retina. To interfere acutely with the interaction of native complexins with the SNARE complex, a peptide consisting of the highly conserved SNARE-binding domain of Cplx3 was introduced via a whole-cell patch pipette placed directly on the synaptic terminal, and vesicle fusion was monitored using capacitance measurements and FM-dye destaining. The inhibitory peptide, but not control peptides, increased spontaneous synaptic vesicle fusion, partially depleted reserve synaptic vesicles, and reduced fusion triggered by opening voltage-gated calcium channels under voltage clamp, without affecting the number of synaptic vesicles a...Continue Reading

Citations

Aug 8, 2015·Cellular and Molecular Life Sciences : CMLS·Ralf MohrmannDieter Bruns
Aug 2, 2018·Physiological Reviews·Tina PangrsicAlexandra Koschak
May 6, 2019·International Journal of Molecular Sciences·Rituparna Chakrabarti, Carolin Wichmann
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May 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Bo ChenRafael L Ufret-Vincenty
Mar 30, 2021·Pflügers Archiv : European journal of physiology·Wallace B Thoreson
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Quirino Attilio VassalliAnnamaria Locascio
Aug 8, 2021·International Journal of Molecular Sciences·Uwe Thorsten LuxJohann Helmut Brandstätter

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