Functional roles of CSPG4/NG2 in chondrosarcoma

International Journal of Experimental Pathology
Nuor S M JamilDonald M Salter

Abstract

CSPG4/NG2 is a multifunctional transmembrane protein with limited distribution in adult tissues including articular cartilage. The purpose of this study was to investigate the possible roles of CSPG4/NG2 in chondrosarcomas and to establish whether this molecule may have potential for targeted therapy. Stable knock-down of CSPG4/NG2 in the JJ012 chondrosarcoma cell line by shRNA resulted in decreased cell proliferation and migration as well as a decrease in gene expression of the MMP (matrix metalloproteinase) 3 protease and ADAMTS4 (aggrecanase). Chondrosarcoma cells in which CSPG4/NG2 was knocked down were more sensitive to doxorubicin than wild-type cells. The results indicate that CSPG4/NG2 has roles in regulating chondrosarcoma cell function in relation to growth, spread and resistance to chemotherapy and that anti-CSPG4/NG2 therapies may have potential in the treatment of surgically unresectable chondrosarcoma.

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Citations

Dec 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elisa TamburiniRoberto Perris
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Theresa D AhrensMette Ø Agerbæk

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