PMID: 11325616Apr 28, 2001Paper

Functional similarities of human and chicken apolipoprotein A-I: dependence on secondary and tertiary rather than primary structure

Biochimica Et Biophysica Acta
Robert S KissG A Francis

Abstract

To investigate the sequence requirements for apolipoprotein (apo) AI functions, comparisons of human and chicken apoAI were performed. In lipid binding assays, chicken apoAI was capable of transforming phospholipid vesicles into discoidal bilayer structures, similar in both size and apolipoprotein content to those produced with human apoAI under the same conditions. Human and chicken apoAI were indistinguishable in their relative abilities to prevent phospholipase C-induced aggregation of human low density lipoprotein. This activity, which is dependent upon formation of a stable interaction with the modified lipoprotein, represents a sensitive measure of apolipoprotein association with spherical lipoprotein particles. The ability of chicken versus human apoAI to mobilize the regulatory pool of cholesterol available for esterification by acyl-CoA:cholesterol acyltransferase by human fibroblasts was also assessed. Lipid-free chicken and human apoAI were equivalent in their ability to deplete cholesterol from this pool, as were intact chicken high density lipoprotein (HDL) and human HDL(3). Based on the overall sequence identity of chicken and human apoAI (48%), and comparison of regions thought to be responsible for key apoAI fun...Continue Reading

References

Feb 20, 1976·Biochimica Et Biophysica Acta·R L JacksonA R Means
Oct 1, 1978·Analytical Biochemistry·A Hara, N S Radin
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A G SuitsJ W Heinecke
May 1, 1974·Analytical Biochemistry·C A NelsonM D Morris
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P E Fielding, C J Fielding
Apr 1, 1982·Journal of Biochemistry·S YokoyamaA Yamamoto
Jul 1, 1995·The Journal of Clinical Investigation·G A FrancisJ F Oram
May 17, 1995·Biochimica Et Biophysica Acta·C G Brouillette, G M Anantharamaiah
Oct 1, 1994·The Journal of Clinical Investigation·A J MendezJ F Oram
Apr 1, 1994·Computer Applications in the Biosciences : CABIOS·D S WishartB D Sykes
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G A FrancisJ W Heinecke
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·W S DavidsonA Jonas
Dec 27, 1996·The Journal of Biological Chemistry·D SviridovN Fidge
Aug 22, 1997·The Journal of Biological Chemistry·Y JiA R Tall
Aug 1, 1997·Cellular and Molecular Life Sciences : CMLS·I CallebautJ P Mornon
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·D W BorhaniC G Brouillette
Aug 4, 1999·Nature Genetics·S G Young, C J Fielding
Oct 8, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·G A Francis, R J Perry

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Citations

Apr 9, 2009·Veterinary Clinical Pathology·Yannick RomanMichel Saint Jalme
Jan 26, 2021·Frontiers in Cell and Developmental Biology·Ting LiuHua Tang
Sep 23, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Vasanthy NarayanaswamiPaul M M Weers

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