Functional skeletal muscle regeneration from differentiating embryonic stem cells.

Nature Medicine
Radbod DarabiRita C R Perlingeiro

Abstract

Little progress has been made toward the use of embryonic stem (ES) cells to study and isolate skeletal muscle progenitors. This is due to the paucity of paraxial mesoderm formation during embryoid body (EB) in vitro differentiation and to the lack of reliable identification and isolation criteria for skeletal muscle precursors. Here we show that expression of the transcription factor Pax3 during embryoid body differentiation enhances both paraxial mesoderm formation and the myogenic potential of the cells within this population. Transplantation of Pax3-induced cells results in teratomas, however, indicating the presence of residual undifferentiated cells. By sorting for the PDGF-alpha receptor, a marker of paraxial mesoderm, and for the absence of Flk-1, a marker of lateral plate mesoderm, we derive a cell population from differentiating ES cell cultures that has substantial muscle regeneration potential. Intramuscular and systemic transplantation of these cells into dystrophic mice results in extensive engraftment of adult myofibers with enhanced contractile function without the formation of teratomas. These data demonstrate the therapeutic potential of ES cells in muscular dystrophy.

References

May 1, 1995·Anatomy and Embryology·B Christ, C P Ordahl
Sep 28, 1995·The New England Journal of Medicine·J R MendellR Rice
Jun 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N TakakuraS Nishikawa
Sep 10, 1998·The Journal of Cell Biology·Z QuJ Huard
Nov 11, 1998·Nature Medicine·T PartridgeE Hoffman
Dec 21, 2000·The Journal of Cell Biology·J R BeauchampP S Zammit
Mar 23, 2001·The Journal of Biological Chemistry·A G Ridgeway, I S Skerjanc
Nov 8, 2001·Current Opinion in Cell Biology·P BaileyA B Lassar
Oct 16, 2002·The Journal of Cell Biology·Atsushi AsakuraMichael A Rudnicki
Oct 29, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Madoka YoshimuraShin'ichi Takeda
Feb 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Smadar Eventov-FriedmanYair Reisner
Apr 22, 2005·Nature·Frédéric RelaixMargaret Buckingham
Jun 11, 2005·Biochemical and Biophysical Research Communications·Satyakam Bhagavati, Weimin Xu
Jun 21, 2005·Genes & Development·Lina Kassar-DuchossoyShahragim Tajbakhsh
Sep 6, 2005·Science·Didier MontarrasMargaret Buckingham
Apr 25, 2006·Muscle & Nerve·Estanislao BachrachLouis M Kunkel
Nov 17, 2006·Nature·Maurilio SampaolesiGiulio Cossu
Apr 10, 2007·Nature Medicine·Tiziano BarberiLorenz Studer
Jun 2, 2007·Cell·Shihuan KuangMichael A Rudnicki
Nov 22, 2007·Science·Junying YuJames A Thomson

❮ Previous
Next ❯

Citations

Feb 22, 2011·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ilaria V LibaniLuisa Ottobrini
Mar 13, 2010·International Journal of Hematology·Michinori Kitagawa, Takumi Era
Jun 27, 2009·Expert Reviews in Molecular Medicine·Lindsey A Muir, Jeffrey S Chamberlain
Dec 10, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yasuhiro KazukiMitsuo Oshimura
Feb 4, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·In-Hyun Park
Jan 27, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Manuel A F V GonçalvesAntoine A F de Vries
Nov 8, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jean-Thomas Vilquin
Mar 7, 2013·Nature Communications·Antonio FilaretoRita C R Perlingeiro
Jul 15, 2009·Stem Cells and Development·Zhifang QiuEn-Kui Duan
Jun 16, 2012·Tissue Engineering. Part B, Reviews·Paola L Carvajal MonroyJohannes W Von den Hoff
May 18, 2013·Tissue Engineering. Part B, Reviews·Jonathan Mark FishmanPaolo De Coppi
May 7, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yi JiaConstance Tom Noguchi
Feb 27, 2009·The Journal of Cardiovascular Nursing·Jesse K Biehl, Brenda Russell
Jul 6, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jennifer L Shadrach, Amy J Wagers
Jun 30, 2012·Science Translational Medicine·Francesco Saverio TedescoGiulio Cossu
Jan 7, 2010·The Journal of Clinical Investigation·Francesco Saverio TedescoGiulio Cossu
Oct 2, 2012·The Journal of Clinical Investigation·Peng HongM A Q Siddiqui
Jul 14, 2010·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Mirella MeregalliYvan Torrente
May 25, 2011·World Journal of Stem Cells·Jean-Marie RamirezJohn De Vos
Dec 1, 2012·Stem Cells Translational Medicine·Karlijn J WilschutHarold S Bernstein
Jul 6, 2013·Regenerative Medicine·Sarah L Parker, Rita C R Perlingeiro
Oct 31, 2012·Journal of the Neurological Sciences·Dimitrios KarussisIbrahim Kassis
Aug 28, 2014·Regenerative Medicine·Jonathan M Van Dyke, Masatoshi Suzuki
Nov 6, 2012·Annual Review of Pathology·Mohammadsharif TabebordbarAmy J Wagers
Jun 19, 2014·Stem Cells and Development·Karolina ArchackaJerzy Moraczewski
Jul 9, 2008·Stem Cell Reviews·Radbod DarabiRita C R Perlingeiro
Apr 5, 2011·Stem Cell Reviews and Reports·Radbod DarabiRita C R Perlingeiro
Jan 27, 2012·Stem Cell Reviews and Reports·Arif AzizF Jeffrey Dilworth
Aug 5, 2015·Journal of Clinical Medicine·Isart RocaAna Belén Alvarez-Palomo
Dec 10, 2015·Stem Cells and Development·Areta M CzerwinskaMaria Anna Ciemerych
Dec 5, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Fabrizio Rinaldi, Rita C R Perlingeiro
Jul 23, 2014·Advanced Drug Delivery Reviews·Karl J A McCullagh, Rita C R Perlingeiro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.