Functional specialisation of yeast Rho1 GTP exchange factors

Journal of Cell Science
Sue Ann KrauseJoseph V Gray

Abstract

Rho GTPases are regulated in complex spatiotemporal patterns that might be dependent, in part at least, on the multiplicity of their GTP exchange factors (GEFs). Here, we examine the extent of and basis for functional specialisation of the Rom2 and Tus1 GEFs that activate the yeast Rho1 GTPase, the orthologue of mammalian RhoA. First, we find that these GEFs selectively activate different Rho1-effector branches. Second, the synthetic genetic networks around ROM2 and TUS1 confirm very different global in vivo roles for these GEFs. Third, the GEFs are not functionally interchangeable: Tus1 cannot replace the essential role of Rom2, even when overexpressed. Fourth, we find that Rom2 and Tus1 localise differently: Rom2 to the growing bud surface and to the bud neck at cytokinesis; Tus1 only to the bud neck, but in a distinct pattern. Finally, we find that these GEFs are dependent on different protein co-factors: Rom2 function and localisation is largely dependent on Ack1, a SEL1-domain-containing protein; Tus1 function and localisation is largely dependent on the Tus1-interacting protein Ypl066w (which we name Rgl1). We have revealed a surprising level of diversity among the Rho1 GEFs that contributes another level of complexity to...Continue Reading

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Citations

Oct 17, 2012·PloS One·Daniel LockshonBrian K Kennedy
Oct 30, 2013·Journal of Cellular Biochemistry·Bo HuangPing-Kun Zhou
Dec 21, 2013·FEMS Microbiology Reviews·Sophie G Martin, Robert A Arkowitz
Dec 5, 2014·Molecular Biology of the Cell·Reshma DavidsonJian-Qiu Wu
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Oct 27, 2017·FEMS Microbiology Reviews·Jürgen J Heinisch, Rosaura Rodicio
Jul 26, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Elena Jiménez-GutiérrezHumberto Martín
Aug 30, 2020·Applied and Environmental Microbiology·Todsapol TechoChoowong Auesukaree
Mar 21, 2020·International Journal of Biological Macromolecules·Edson Mario de Andrade SilvaFabienne Micheli
Jan 7, 2022·World Journal of Microbiology & Biotechnology·Lingjie ZengYi Yi

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