PMID: 9660870Jul 14, 1998Paper

Functional specialization of stable and dynamic microtubules in protein traffic in WIF-B cells

The Journal of Cell Biology
C PoüsG Durand

Abstract

We found that the magnesium salt of ilimaquinone, named 201-F, specifically disassembled dynamically unstable microtubules in fibroblasts and various epithelial cell lines. Unlike classical tubulin- interacting drugs such as nocodazole or colchicine which affect all classes of microtubules, 201-F did not depolymerize stable microtubules. In WIF-B-polarized hepatic cells, 201-F disrupted the Golgi complex and inhibited albumin and alpha1-antitrypsin secretion to the same extent as nocodazole. By contrast, 201-F did not impair the transport of membrane proteins to the basolateral surface, which was only affected by the total disassembly of cellular microtubules. Transcytosis of two apical membrane proteins-the alkaline phosphodiesterase B10 and dipeptidyl peptidase IV-was affected to the same extent by 201-F and nocodazole. Taken together, these results indicate that only dynamically unstable microtubules are involved in the transport of secretory proteins to the plasma membrane, and in the transcytosis of membrane proteins to the apical surface. By contrast, stable microtubules, which are not functionally affected by 201-F treatment, are involved in the transport of membrane proteins to the basolateral surface. By specifically d...Continue Reading

References

Apr 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L Saucan, G E Palade
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·W BollJ D Jamieson
Jan 1, 1991·Annual Review of Cell Biology·V I Gelfand, A D Bershadsky
Jan 5, 1990·Science·B EddéP Denoulet
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M LeDizet, G Piperno
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D R WebsterG G Borisy
Apr 1, 1985·The Journal of Cell Biology·A L HubbardL T Braiterman
Jul 30, 1982·Biochemical and Biophysical Research Communications·K Oda, Y Ikehara
Sep 1, 1983·The Journal of Cell Biology·M Pavelka, A Ellinger
Feb 1, 1982·Archives of Disease in Childhood·M MäkiJ K Visakorpi
Sep 1, 1984·The Journal of Cell Biology·A A Rogalski, S J Singer
Dec 1, 1993·The Journal of Cell Biology·G IhrkeA L Hubbard
Feb 1, 1995·Current Opinion in Cell Biology·N B Cole, J Lippincott-Schwartz
Jan 1, 1995·The Journal of Cell Biology·E TanakaM W Kirschner
Mar 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M MauriceA L Hubbard
Feb 1, 1994·Current Opinion in Cell Biology·N Hirokawa
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G Gurland, G G Gundersen
Mar 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J D Moore, S A Endow
Feb 1, 1997·Current Opinion in Cell Biology·H V GoodsonT E Kreis
Apr 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L J ScottM Maurice
Mar 1, 1997·European Journal of Biochemistry·T H MacRae

❮ Previous
Next ❯

Citations

Jul 28, 1999·Cell Motility and the Cytoskeleton·A di CampliG Egea
Nov 6, 2009·Genes & Nutrition·Blythe D ShepardPamela L Tuma
Jun 12, 2003·Experimental Cell Research·Véronique CrucianiSvein Ole Mikalsen
May 29, 2002·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·J S Y Lee, A I Gotlieb
May 21, 2010·The Journal of Biological Chemistry·Camille GeeraertChristian Poüs
Jul 14, 2001·Molecular Biology of the Cell·K Chabin-BrionC Poüs
Mar 3, 2006·BMC Cell Biology·Andrei I IvanovCharles A Parkos
Mar 18, 2009·World Journal of Gastroenterology : WJG·Blythe D Shepard, Pamela L Tuma
Mar 20, 2010·World Journal of Gastroenterology : WJG·Blythe D Shepard, Pamela L Tuma
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·P ZhengS W Pimplikar
Aug 26, 2014·Molecular and Cellular Biochemistry·Jennifer L GroebnerPamela L Tuma
Sep 24, 2015·Biomolecules·Jennifer L Groebner, Pamela L Tuma
Apr 12, 2011·Expert Review of Gastroenterology & Hepatology·Agnes MolnarPavel Strnad
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V BenderD Cassio
Dec 29, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rohan A JosephPamela L Tuma
Jun 14, 2008·Biology of the Cell·Catherine DecaensDoris Cassio
Sep 20, 2005·Journal of Hepatology·George T KannarkatPamela L Tuma
Jun 28, 2015·Journal of Hepatology·Paul Gissen, Irwin M Arias
Jun 16, 2004·Developmental Biology·Juan FernándezAndrea Ubilla
Nov 1, 2016·Cell Structure and Function·Mika Toya, Masatoshi Takeichi
Aug 11, 2006·The Journal of Biological Chemistry·Tsutomu NakagawaEiji Miyoshi
Nov 6, 2004·The Journal of Biological Chemistry·Thu Phung-KoskasAnne Drechou
Apr 10, 2020·Cell Structure and Function·Yutaka TakedaDaiju Kitagawa
Jul 5, 2003·Physiological Reviews·Pamela L Tuma, Ann L Hubbard
Feb 15, 2001·American Journal of Physiology. Cell Physiology·C Decaens, D Cassio
Dec 22, 1999·The American Journal of Physiology·N O KuM B Omary
Apr 27, 2013·Journal of Cell Science·Rafah MackehChristian Poüs
Jun 5, 2021·Nature Chemical Biology·Nandini ManiRadhika Subramanian
Oct 10, 2002·Journal of the American Chemical Society·Taotao LingEmmanuel A Theodorakis

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