PMID: 8971810Nov 29, 1996Paper

Functional studies of single-site variants in the calmodulin-binding domain of RC3/neurogranin in Xenopus oocytes

Neuroscience Letters
J B WatsonR W Cohen

Abstract

Single-site variants in the calmodulin-binding domain of RC3/neurogranin were heterologously expressed in Xenopus oocytes to examine their effects on serotonin-evoked currents. RC3 variants serine36 -->alanine (Ser36-->Ala), serine36-->glycine (Ser36-->Gly), and phenylalanine37-->tryptophan (Phe37-->Trp), which bind calmodulin but are deficient in protein kinase C (PKC) phosphorylation, display serotonin-evoked Ca(2+)-dependent Cl- currents in oocytes similar to control oocytes. A serine36-->aspartate (Ser36-->Asp) variant, which does not bind calmodulin and mimics the PKC-phosphorylated state of RC3, significantly enhances serotonin-evoked currents in a manner similar to wild-type. The results suggest that RC3 not only regulates the availability of free calmodulin in a dendritic spine but also, when phosphorylated, independently stimulates G-protein coupled second messenger pathways that generate inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) and intracellular Ca2+.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J B WatsonR S Fisher
Feb 1, 1992·Current Opinion in Cell Biology·R E Cheney, M S Mooseker
Aug 1, 1990·Journal of Neuroscience Research·J B WatsonJ G Sutcliffe
Jan 1, 1989·Annual Review of Neuroscience·J H Skene
Jan 1, 1994·Journal of Molecular Neuroscience : MN·D D GerendasyJ G Sutcliffe
Dec 1, 1994·Brain Research. Molecular Brain Research·J B WatsonP M Coulter
Sep 1, 1993·Archives of Biochemistry and Biophysics·K P HuangH C Chen
Jun 1, 1993·Trends in Pharmacological Sciences·P P HumphreyD Hoyer
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E KlannJ D Sweatt

❮ Previous
Next ❯

Citations

Dec 13, 1997·Molecular Neurobiology·D D Gerendasy, J G Sutcliffe
Jul 16, 2003·The European Journal of Neuroscience·Jacqueline J W van DalenDonna L Gruol
Aug 15, 2006·Pharmacology & Therapeutics·Hey-Kyoung Lee
Dec 26, 2006·Neurobiology of Disease·Silvana FranceschettiMichele Simonato
Feb 14, 2007·The Biochemical Journal·Irene Domínguez-GonzálezF Javier Díez-Guerra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.