Functional studies of the gene slr2049 from Synechocystis sp. PCC6803 and its site-directed mutation

Gene
Bingjun LiuLei Zhang

Abstract

Phycobiliprotein is a homologous family of light-harvesting chromoproteins existing in cyanobacteria, red algae and cryptophytes. Phycobiliprotein is made up of phycobilin and its corresponding apophycobiliprotein, and they are covalently linked by the thioether bond with the bilin lyase. Using the software BLAST, we have found gene slr2049 in Synechocystis sp. PCC6803 homologous to the biliprotein lyase gene cpeS. This paper investigates the protein expressed by gene slr2049 to find the enzymatic activity characteristics. We cloned slr2049 and its related genes cpcB, ho1, and pcyA which are linked with the synthesis of phycocyanin. Special amino acid mutagenesis was performed on slr2049 to construct eight mutants slr2049 (H21S), slr2049 (L23S), slr2049 (A24S), slr2049 (F25S), slr2049 (W72L), slr2049 (G84S), slr2049 (R107S) and slr2049 (Y124S). These mutants were ligated with vectors pEDFDuet-1 and pET-23a to construct pCDF-cpcB-slr2049 wild-type, pCDF-cpcB-slr2049 mutants and pET-ho1-pcyA, for the purpose of protein expression and analysis. The results showed that the wild-type and mutants slr2049 (H21S), slr2049 (L23S), slr2049 (F25S), slr2049 (W72L), slr2049 (G84S), and slr2049 (Y124S) can catalyze CpcB to couple on PCB corr...Continue Reading

References

Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·A N Glazer
Mar 12, 1998·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·C RomayI García
Mar 2, 1999·Journal of Structural Biology·R MacColl
Aug 3, 2001·Il Farmaco·J E Piñero EstradaA M Villar del Fresno
Feb 21, 2008·Molecular Microbiology·H Scheer, K-H Zhao
Jul 8, 2010·Photosynthesis Research·Shaofang LiuSong Qin
Jan 27, 2011·Biochemical Society Transactions·Vera L GonçalvesMiguel Teixeira
Jan 1, 1986·Photosynthesis Research·B A Zilinskas, L S Greenwald

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Citations

Mar 18, 2021·PloS One·Apiwat SangphukieoMarasri Ruengjitchatchawalya

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