PMID: 8606186Apr 1, 1996Paper

Functional studies of the gvpACNO operon of Halobacterium salinarium reveal that the GvpC protein shapes gas vesicles

Journal of Bacteriology
S OffnerF Pfeifer

Abstract

Gas vesicle (Vac) synthesis in Halobacterium salinarium PHH1 involves the expression of the plasmid pHH1-encoded vac (p-vac) region consisting of 14 different gvp genes that are arranged in two clusters, p-gvpACNO and, oriented in the direction opposite to that of gvpA, p-gvpDEFGHIJKLM. The p-gvpACNO region was analyzed at the transcriptional and functional levels in H. salinarium and in Haloferax volcanii transformants containing subfragments of the p-vac region. The p-gvpACNO genes were transcribed as several mRNAs: the 270-nucleotide (nt) p-gvpA transcript, encoding the major structural protein, occurred in large amounts, and minor amounts of three different readthrough transcripts (p-gvpACN, and p-gvpACNO mRNA) were found. In addition, the p-gvpO gene gave rise to two separate mRNA species: a 550-nt mRNA starting at the ATG and spanning the entire reading frame and a 420-nt RNA encompassing the second half of the p-gvpO gene. The requirement of p-gvpC, p-gvpN, and p-gvpO gene expression for gas vesicle synthesis was assessed by transformation experiments using the VAC- species Haloferax volcanii as the recipient. A delta C transformant, harboring the p-vac region with a deletion of the p-gvpC gene, produced large amounts of...Continue Reading

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Citations

Mar 27, 2002·DNA Sequence : the Journal of DNA Sequencing and Mapping·D AlbouyN T De Marsac
Sep 4, 2012·Nature Reviews. Microbiology·Felicitas Pfeifer
Jul 5, 2017·BMC Genomics·Henk BolhuisFrancisco Rodriguez-Valera
Jun 14, 2019·Frontiers in Microbiology·Johannes Born, Felicitas Pfeifer
May 1, 2020·Environmental Microbiology·Alex Quintero-YanesGeorge Salmond
Feb 6, 2004·Molecular Microbiology·Simone Sartorius-Neef, Felicitas Pfeifer
Nov 23, 2005·The Journal of Biological Chemistry·Jamie SniderWalid A Houry
Nov 15, 2008·Molecular Microbiology·Torsten Hechler, Felicitas Pfeifer
Jan 9, 2016·Environmental Microbiology·Yosuke TashiroGeorge P C Salmond
Sep 10, 2015·Vaccines·Shiladitya DasSarma, Priya DasSarma
Mar 27, 2018·Annual Review of Chemical and Biomolecular Engineering·David MarescaMikhail G Shapiro
Sep 4, 2018·Frontiers in Microbiology·Kerstin WinterFelicitas Pfeifer
Jan 6, 2005·Microbiology·Dagmar Gregor, Felicitas Pfeifer
Dec 8, 2020·Frontiers in Microbiology·Kerstin VölknerFelicitas Pfeifer
Jun 5, 1998·Microbiology·Sonja OffnerFelicitas Pfeifer
Jun 14, 2001·Systematic and Applied Microbiology·Y Luo, A Wasserfallen
Dec 17, 2010·Journal of Proteome Research·Lichieh Julie ChuWailap Victor Ng
Aug 3, 1999·Genetics·W B WhitmanA Klein

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