Functional studies on human Pex7p: subcellular localization and interaction with proteins containing a peroxisome-targeting signal type 2 and other peroxins

The Biochemical Journal
Karen GhysPaul P Van Veldhoven

Abstract

Pex7p is a WD40-containing protein involved in peroxisomal import of proteins containing an N-terminal peroxisome-targeting signal (PTS2). The interaction of human recombinant Pex7p expressed in different hosts/systems with its PTS2 ligand and other peroxins was analysed using various experimental approaches. Specific binding of human Pex7p to PTS2 could be demonstrated only when Pex7p was formed in vitro by a coupled transcription/translation system or synthesized in vivo in Chinese hamster ovary K1 cells transfected with a construct coding for a Pex7p-green fluorescent protein (GFP) fusion protein. Apparently, no cofactors are required and only monomeric Pex7p binds to PTS2. The interaction is reduced upon cysteine alkylation and is impaired upon truncation of the N-terminus of Pex7p. Interaction of Pex7p with other peroxins could not be demonstrated in bacterial or yeast two-hybrid screens, or in pull-down binding assays. The GFP fusion proteins, tagged at either the N- or C-terminus, were able to restore PTS2 import in rhizomelic chondrodysplasia punctata fibroblasts, and Pex7p-GFP was located both in the lumen of peroxisomes and in the cytosol.

Citations

Dec 21, 2006·Histochemistry and Cell Biology·Werner J KovacsSkaidrite K Krisans
Aug 2, 2003·Current Opinion in Cell Biology·Paul B Lazarow
Nov 8, 2011·The Journal of Biological Chemistry·Markus KunzeJohannes Berger
Jan 1, 2009·BMJ Case Reports·Sofie J HuybrechtsDavid Cassiman
Oct 7, 2003·Annual Review of Genomics and Human Genetics·Sabine WellerDavid Valle
Nov 17, 2004·The Journal of Cell Biology·Devi M NairPaul B Lazarow
Feb 3, 2004·Journal of Cellular and Molecular Medicine·Laura-Anne Brown, Alison Baker
Mar 19, 2014·The Biochemical Journal·Ana Victoria C PilarArmando Jardim
Nov 13, 2004·FEMS Microbiology Reviews·Juliette MoyersoenPaul A M Michels
Sep 26, 2006·Biochimica Et Biophysica Acta·Paul B Lazarow
Aug 9, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Tânia FranciscoJorge E Azevedo
Aug 2, 2013·The Journal of Biological Chemistry·Changle MaSuresh Subramani
Jan 13, 2004·The Journal of Biological Chemistry·Marc FransenPaul P Van Veldhoven
Apr 9, 2020·Frontiers in Cell and Developmental Biology·Paul P Van VeldhovenElke Van Ael
May 21, 2009·IUBMB Life·Changle Ma, Suresh Subramani
Nov 22, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Markus Kunze
Jan 20, 2004·Journal of Cell Science·Sietske HogenboomHans R Waterham

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