PMID: 11341969May 9, 2001Paper

Functional testing of putative oligopeptide permease (Opp) proteins of Borrelia burgdorferi: a complementation model in opp(-) Escherichia coli

Biochimica Et Biophysica Acta
Bo LinL T Hu

Abstract

Studies of the protein function of Borrelia burgdorferi have been limited by a lack of tools for manipulating borrelial DNA. We devised a system to study the function of a B. burgdorferi oligopeptide permease (Opp) orthologue by complementation with Escherichia coli Opp proteins. The Opp system of E. coli has been extensively studied and has well defined substrate specificities. The system is of interest in B. burgdorferi because analysis of its genome has revealed little identifiable machinery for synthesis or transport of amino acids and only a single intact peptide transporter operon. As such, peptide uptake may play a major role in nutrition for the organism. Substrate specificity for ABC peptide transporters in other organisms is determined by their substrate binding protein. The B. burgdorferi Opp operon differs from the E. coli Opp operon in that it has three separate substrate binding proteins, OppA-1, -2 and -3. In addition, B. burgdorferi has two OppA orthologues, OppA-4 and -5, encoded on separate plasmids. The substrate binding proteins interact with integral membrane proteins, OppB and OppC, to transport peptides into the cell. The process is driven by two ATP binding proteins, OppD and OppF. Using opp-deleted E. c...Continue Reading

References

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Citations

Aug 28, 2003·Microbes and Infection·Brian StevensonKelly Babb
Oct 27, 2010·Infection and Immunity·A Kovacs-SimonS L Michell
Jan 24, 2007·Journal of Bacteriology·Melisa S MedranoLinden T Hu
Aug 23, 2011·Journal of Bacteriology·Ozan S KumruWolfram R Zückert
Apr 3, 2004·Journal of Bacteriology·Dionysios LiverisIra Schwartz
Dec 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sukanya NarasimhanMelissa J Camaino
Dec 27, 2005·FEBS Letters·Esther Biemans-OldehinkelBert Poolman
Mar 14, 2014·Clinical and Vaccine Immunology : CVI·Giacomo SignorinoRaymond J Dattwyler

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