Functional Thy-1+ cells in cultures of spleen cells from nu/nu mice

European Journal of Immunology
G Dennert, R Hyman

Abstract

Thy-1+ lymphocytes, detectable by quantitative serum absorption, arise in cultures of spleen cells from congenitally athymic (nu/nu) mice supplemented with supernatants from cultures of normal spleen cells stimulated with the T cell mitogen concanavalin A. Pretreatment of nu/nu spleen cells with appropriate anti-Thy-1 alloantibody and complement prior to culture reduces their capacity to generate Thy-1+ cells by about 90%. This shows that the majority of cells proliferating in these cultures are descendants of Thy-1+ cells which can be detected in the original nu/nu spleens. Experiments aimed at exploring the function of these Thy-1+ cells after culture in conditioned medium revealed that within one or two days after culture initiation, strong cooperative activity for a humoral response to xenogeneic erythrocytes can be detected. In mixtures of bone marrow-derived lymphocytes from various mouse strains with cultured nu/nu spleen cells, it was observed that T-B cooperation is not H-2-restricted. Attempts at inducing T cell-mediated cytotoxicity to alloantigens in such cultures of nu/nu spleen cells were unsuccessful. In contrast, nonspecific cytotoxicity which was attributed to natural killer cells was regularly observed and cou...Continue Reading

References

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Citations

Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J S Lipsick, N O Kaplan
Mar 1, 1981·The Journal of Experimental Medicine·G DennertS Yamagata
Jul 1, 1983·Annales d'immunologie·J J OwenR Kingston
Sep 1, 1988·European Journal of Cancer & Clinical Oncology·L M Ching, B C Baguley
Oct 1, 1983·Scandinavian Journal of Immunology·K O GrönvikP Stern
Sep 1, 1983·European Journal of Immunology·N ChristensenJ Marbrook
Nov 1, 1985·European Journal of Immunology·I J WeilerE Weiler

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