Functional transplantation of the sumoylation machinery into Escherichia coli

Protein Expression and Purification
Mario Mencía, Víctor de Lorenzo

Abstract

Modification by SUMO proteins appears to be very common in eukaryotic cells. Many proteins have been reported to be sumoylated, at least under certain circumstances, in vivo, and new examples get published every month. On the other hand, sumoylation is, in essence, a way to construct branched proteins or protein fusions. Obtention of pure sumoylated proteins from eukaryotic cells is not easy because of the dynamic nature of this modification and the large number of sumoylated proteins in vivo. Production of sumoylated proteins in vitro requires the previous purification of most of the components of the pathway, and has the typical limitations of such systems. In this paper, we describe a method to quantitatively produce sumoylated proteins in vivo in Escherichia coli as a way to obtain large quantities of specifically sumoylated target proteins with a high degree of purity, to generate fusion proteins not limited to N- or C-end additions, and to polymerize proteins by covalent linkage.

References

Oct 27, 1997·The Journal of Biological Chemistry·E S Johnson, G Blobel
Apr 3, 1999·The Journal of Biological Chemistry·J M DesterroR T Hay
Feb 29, 2000·The Journal of Biological Chemistry·H Saitoh, J Hinchey
May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A CiechanoverA L Schwartz
Jan 2, 2001·The Journal of Biological Chemistry·M S RodriguezR T Hay
Jul 14, 2001·The Journal of Biological Chemistry·M H TathamR T Hay
May 16, 2002·Traffic·Andrea Pichler, Frauke Melchior
May 16, 2002·Journal of Cellular Physiology·Keun Il KimChin Ha Chung
Jun 22, 2002·Molecular and Cellular Biology·Noora KotajaJorma J Palvimo
Jul 19, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Christopher H Eskiw, David P Bazett-Jones
Jun 27, 2003·Trends in Biochemical Sciences·David C Schwartz, Mark Hochstrasser
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·Jacob-S Seeler, Anne Dejean
Nov 11, 2003·Trends in Biochemical Sciences·Frauke MelchiorAndrea Pichler

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Citations

Dec 4, 2004·Molecular & Cellular Proteomics : MCP·Germán Rosas-AcostaVan G Wilson
Dec 4, 2008·Nucleic Acids Research·Laura M GreenStefan G E Roberts
Feb 24, 2010·Molecular and Cellular Biology·Shen-hsi Yang, Andrew D Sharrocks
Dec 14, 2011·PloS One·Javier GarzónPilar Sánchez-Blázquez
Jan 9, 2007·Journal of Virological Methods·Martijn A LangereisVan G Wilson
Nov 15, 2011·The EMBO Journal·Tal Keren-KaplanGali Prag
Nov 21, 2012·Journal of Integrative Plant Biology·Gema López-TorrejónJuan C del Pozo
Mar 27, 2010·The Biochemical Journal·James WittyAndrew D Sharrocks
Jun 29, 2011·Protein Expression and Purification·Zoé LensAlexis Verger
Oct 1, 2013·Methods : a Companion to Methods in Enzymology·Sayanti Saha, Jonathan Chernoff
Mar 7, 2012·Journal of Molecular Biology·Gurkan Guntas, Brian Kuhlman
Dec 9, 2014·The Journal of Clinical Investigation·Klaus-Dieter PreussBoris Kubuschok
Jun 15, 2017·Wellcome Open Research·Elisa Aguilar-MartinezAndrew D Sharrocks
Jul 31, 2019·ACS Chemical Biology·Yuqing JiaHuib Ovaa

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