Functional up-regulation of basolateral Na+-dependent HCO3- transporter NBCn1 in medullary thick ascending limb of K+-depleted rats

Pflügers Archiv : European journal of physiology
Jakob K JakobsenJens Leipziger

Abstract

The electroneutral, Na(+)-coupled HCO(3)(-) co-transporter (NBCn1) is expressed in the basolateral membrane of rat medullary thick ascending limb (mTAL) segments and is up-regulated strongly during metabolic acidosis. We have suggested previously that NBCn1-mediated HCO(3)(-) uptake across the basolateral cell membrane is an important buffering mechanism during the transcellular transport of NH(4)(+) in the mTAL. To investigate this further we treated rats with a low-K(+) diet for 4 days, a manoeuvre known to increase proximal tubular ammoniagenesis and delivery of luminal NH(4)(+) to the mTAL. Hypokalaemia strongly increased immunolabelling of basolateral NBCn1 in the mTAL of the inner stripe of the outer medulla. Immunoblotting revealed an eightfold up-regulation of NBCn1 protein in K(+)-depleted rats. Subsequently we used in vitro perfusion of isolated mTAL and BCECF imaging to investigate Na(+)-coupled HCO(3)(-) influx in normal and K(+)-depleted rats. Hypokalaemia induced a threefold up-regulation of Na(+)-coupled HCO(3)(-) influx (1.64+/-0.28 vs. 0.47+/-0.05 pH units/minute, n=8). The Na(+)-dependent alkalinization was also significantly larger in K(+)-depleted rats (0.38+/-0.04 vs. 0.23+/-0.03 pH units). These data indic...Continue Reading

References

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Citations

Nov 21, 2007·Pflügers Archiv : European journal of physiology·C S GeytiH A Praetorius
Mar 12, 2009·Pflügers Archiv : European journal of physiology·Carsten A WagnerNilufar Mohebbi
Dec 2, 2010·Cell and Tissue Research·Eleni Roussa
Jun 6, 2009·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Guixian WangJørgen Frøkiaer
May 21, 2010·The Journal of Clinical Investigation·Soline BourgeoisPascal Houillier
Nov 28, 2017·Journal of Nephrology·Francesco TrepiccioneSebastian Frische
Feb 3, 2017·Physiological Reviews·I David Weiner, Jill W Verlander
Jul 28, 2005·Current Opinion in Nephrology and Hypertension·Michael F Romero
Feb 8, 2006·American Journal of Physiology. Renal Physiology·Alexander Pushkin, Ira Kurtz
Apr 17, 2013·Physiological Reviews·Mark D Parker, W F Boron
Jul 11, 2015·American Journal of Physiology. Renal Physiology·Carsten A WagnerSoline Bourgeois
Dec 14, 2007·American Journal of Physiology. Cell Physiology·Ebbe BoedtkjerChristian Aalkjaer

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