PMID: 6408480Jun 30, 1983Paper

Functional V region formation during in vitro culture of a murine immature B precursor cell line

Nature
H SugiyamaT Kishimoto

Abstract

B lymphocytes originate from pluripotential haematopoietic stem cells and differentiate into immunoglobulin (Ig)-producing cells. B-cell lineage differentiation is accompanied by two types of immunoglobulin gene rearrangements--rearrangement of V, D and J gene segments to create a functional V gene and rearrangement of CH genes for heavy-chain switching. These results, however, have been obtained mainly by analysis of immunoglobulin gene organization of myeloma cells. Baltimore and his colleagues have established Abelson murine leukaemia virus (A-MuLV)-transformed cell lines and found a few lines capable of carrying out kappa-gene rearrangement or undergoing isotype switching during in vitro culture. To study early B-cell lineage differentiation events, we have now also established A-MuLV-transformed cell lines which are capable of differentiating from mu- to mu+ and of undergoing continuing rearrangement of heavy-chain genes in culture. Analysis of immunoglobulin gene organization of these transformed cells revealed that mu- cells have already undergone DNA rearrangements involving JH segments but an additional rearrangement of JH segments is required for initiation of mu-chain synthesis. Southern blot analysis of the DNA and ...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T Honjo, T Kataoka
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C Nottenburg, I L Weissman
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Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R P PerryJ F Kearney

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Citations

Feb 1, 1988·Molecular Immunology·Y TaniS Kishimoto
Jul 1, 1997·Molecular Immunology·C F WebbE A Smith
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M HagiyaH Sakano
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H KubagawaP D Burrows
Oct 1, 1984·The Journal of Experimental Medicine·K A DenisO N Witte
Aug 1, 1987·The Journal of Experimental Medicine·H SugiyamaN Sakato
Jun 1, 1995·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·T Komori, H Sugiyama
Nov 1, 1984·The Journal of Clinical Investigation·R FoaF Lauria
Feb 1, 1993·European Journal of Immunology·T KomoriH Sugiyama
Jan 1, 1984·Cancer Investigation·M Potter, J F Mushinski
Sep 1, 1983·Cell·S AkiraT Kishimoto
Nov 20, 1987·Science·F W AltG D Yancopoulos

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