Functional Validation of Two Fungal Subfamilies in Carbohydrate Esterase Family 1 by Biochemical Characterization of Esterases From Uncharacterized Branches

Frontiers in Bioengineering and Biotechnology
Xinxin LiAdiphol Dilokpimol

Abstract

The fungal members of Carbohydrate Esterase family 1 (CE1) from the CAZy database include both acetyl xylan esterases (AXEs) and feruloyl esterases (FAEs). AXEs and FAEs are essential auxiliary enzymes to unlock the full potential of feedstock. They are being used in many biotechnology applications including food and feed, pulp and paper, and biomass valorization. AXEs catalyze the hydrolysis of acetyl group from xylan, while FAEs release ferulic and other hydroxycinnamic acids from xylan and pectin. Previously, we reported a phylogenetic analysis for the fungal members of CE1, establishing five subfamilies (CE1_SF1-SF5). Currently, the characterized AXEs are in the subfamily CE1_SF1, whereas CE1_SF2 contains mainly characterized FAEs. These two subfamilies are more related to each other than to the other subfamilies and are predicted to have evolved from a common ancestor, but target substrates with a different molecular structure. In this study, four ascomycete enzymes from CE1_SF1 and SF2 were heterologously produced in Pichia pastoris and characterized with respect to their biochemical properties and substrate preference toward different model and plant biomass substrates. The selected enzymes from CE1_SF1 only exhibited AX...Continue Reading

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Citations

Jan 14, 2021·Journal of Fungi·Mariane DaouCraig B Faulds
Jul 24, 2021·Carbohydrate Polymers·Mingjun ZhangJianbin Li
Aug 12, 2021·Journal of Bioscience and Bioengineering·Tomoe KatoTakuya Koseki

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Methods Mentioned

BETA
glycosylation
acetylation

Software Mentioned

Molecular Evolutionary Genetic Analysis software
Easy Sequencing
MEGA7
ImageJ
SignalP
Sequence Manipulation Suite
NetNGlyc
BLAST

Related Concepts

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