Functionality of lactic acid bacteria peptidase activities in the hydrolysis of gliadin-like fragments

Letters in Applied Microbiology
C L GerezG C Rollán

Abstract

To evaluate the role of the peptidase activities from sourdough lactic acid bacteria (LAB) in the degradation of alpha-gliadin fragments. Different proline-containing substrates were hydrolysed by LAB indicating pro-specific peptidase activities. Lactobacillus plantarum CRL 775 and Pediococcus pentosaceus CRL 792 displayed the highest tri- and di-peptidase activities, respectively. Lactobacillus plantarum strains hydrolysed more than 60%alpha-gliadin fragments corresponding to the 31-43 and 62-75 amino acids in the protein after 2 h. None of the LAB strains alone could hydrolyse 57-89 alpha-gliadin peptide; however, the combination of L. plantarum CRL 775 and P. pentosaceus CRL 792 led to hydrolysis (57%) of this peptide in 8 h. The capacity of LAB strains to degrade alpha-gliadin fragments was not correlated to individual peptidase activities. Several strains separately degraded the 31-43 and 62-75 alpha-gliadin fragments, while the 57-89 peptide degradation was associated with the combination of peptidase profiles from pooled LAB strains. This is the first report on the peptide hydrolase system of sourdough pediococci and its ability to reduce alpha-gliadin fragments. This study contributes to a better knowledge of sourdough ...Continue Reading

References

Jan 5, 2002·International Journal of Food Microbiology·G Rollan, G F Font de Valdez
Sep 12, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Felix HauschChaitan Khosla
Jun 20, 2003·The New England Journal of Medicine·Markku MäkiMikael Knip
Feb 10, 2004·Applied and Environmental Microbiology·Raffaella Di CagnoMarco Gobbetti
Nov 29, 2005·Biochimica Et Biophysica Acta·Maria De AngelisMarco Gobbetti
Nov 30, 2005·Journal of Applied Microbiology·G RollánG F de Valdez
Apr 20, 2006·Letters in Applied Microbiology·C L GerezG F de Valdez
Jun 24, 2006·Chemistry & Biology·Matthew SiegelChaitan Khosla
Sep 30, 2006·Food Microbiology·Marco GobbettiMaria De Angelis

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Citations

Oct 25, 2013·Nutrients·Isabel CominoCarolina Sousa
Dec 24, 2015·Journal of the Science of Food and Agriculture·Ilona StefańskaKrystyna M Stecka
Oct 5, 2019·Acta Crystallographica. Section F, Structural Biology Communications·Deanna Dahlke OjennusDouglas H Juers
Jul 17, 2012·Journal of Agricultural and Food Chemistry·Cristiana GarofaloFrancesca Clementi

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