Functionalization and peptide-based delivery of magnetic nanoparticles as an intracellular MRI contrast agent

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Nitin NitinGang Bao

Abstract

We report the development of functionalized superparamagnetic iron oxide nanoparticles with a PEG-modified, phospholipid micelle coating, and their delivery into living cells. The size of the coated particles, as determined by dynamic light scattering and electron microscopy, was found to be between 12 and 14 nm. The PEG-phospholipid coating resulted in high water solubility and stability, and the functional groups of modified PEG allowed for bioconjugation of various moieties, including a fluorescent dye and the Tat peptide. Efficient delivery of the functionalized nanoparticles into living cells was confirmed by fluorescence microscopy, relaxation time measurements, and magnetic resonance imaging (MRI). This demonstrates the feasibility of using functionalized magnetic nanoparticles with uniform (approximately 10 nm) sizes as an MRI contrast agent for intracellular molecular imaging in deep tissue. These micelle-coated iron oxide nanoparticles offer a versatile platform for conjugation of a variety of moieties, and their small size confers advantages for intracellular molecular imaging with minimal perturbation.

References

Sep 2, 1999·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·M Jones, J Leroux
Jun 14, 2000·International Journal of Pharmaceutics·W R PerkinsE Mayhew
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·V P TorchilinT S Levchenko
Mar 19, 2002·Advanced Drug Delivery Reviews·Vladimir P Torchilin
Mar 21, 2002·Journal of the American Chemical Society·J Manuel PerezLee Josephson
Dec 3, 2002·Science·Benoit DubertretAlbert Libchaber
Feb 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vladimir P TorchilinGerard G M D'Souza
Feb 13, 2003·Journal of the American Chemical Society·Ansil DyalRichard A Gross
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vladimir P TorchilinBrigitte Papahadjopoulos-Sternberg
Jul 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Devin DressmanBert Vogelstein
Aug 19, 2003·Biomaterials·Ruxandra GrefEvgueni Mysiakine

❮ Previous
Next ❯

Citations

Feb 24, 2006·Annals of Biomedical Engineering·Daniel L J ThorekAndrew Tsourkas
Oct 11, 2011·Annals of Biomedical Engineering·Qin MiaoEric J Seibel
Oct 31, 2012·Science China. Life Sciences·Sheng TongGang Bao
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Kimberly A KellyRalph Weissleder
Jun 27, 2012·Journal of Nanobiotechnology·Clare HoskinsLijun Wang
Aug 9, 2008·International Journal of Nanomedicine·Stuart C McBainJon Dobson
Nov 11, 2008·International Journal of Nanomedicine·Xiang-Hong PengDong M Shin
May 26, 2011·Biomedical Imaging and Intervention Journal·J LodhiaSf Cowell
Jul 13, 2011·International Journal of Molecular Sciences·Daisuke KamiMasashi Toyoda
Nov 16, 2013·International Journal of Molecular Sciences·Bashar IssaYousef Haik
Feb 6, 2013·Theranostics·Brian T LukLiangfang Zhang
Nov 13, 2013·Analytical Biochemistry·Boram Kang, Suk-Jung Choi
Aug 25, 2007·Nanomedicine·Jason R McCarthyRalph Weissleder
Aug 25, 2007·Nanomedicine·Willem J M MulderZahi A Fayad
Dec 4, 2013·Annals of Biomedical Engineering·Sheng TongGang Bao
May 22, 2013·Advances in Colloid and Interface Science·Jacob WeingartXue-Long Sun
Mar 23, 2011·Annual Review of Analytical Chemistry·Jacob S BeveridgeMary Elizabeth Williams
Oct 22, 2011·Cellular and Molecular Life Sciences : CMLS·Paul GalvinRonan MacLoughlin
Jul 31, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Luca Frullano, Thomas J Meade
Oct 5, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·He MengNing Dai
Aug 8, 2008·Journal of Drug Targeting·Rupa R SawantVladimir P Torchilin
Jun 12, 2012·Trends in Molecular Medicine·Erez Koren, Vladimir P Torchilin
Jul 29, 2015·Data in Brief·Priyanka SinghAmnon Kohen
Dec 23, 2008·Biochemical and Biophysical Research Communications·Jin Sook SuhYoon Jeong Park
Jun 10, 2008·International Journal of Pharmaceutics·Guifang HuangLeonard I Wiebe
Jan 24, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vijay A Sethuraman, You Han Bae
Oct 7, 2015·ACS Applied Materials & Interfaces·Chariya KaewsanehaAbdelhamid Elaissari
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Shivang R Dave, Xiaohu Gao
Oct 16, 2012·NMR in Biomedicine·Timo Heidt, Matthias Nahrendorf
Mar 11, 2011·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Leon M BellanRobert S Langer
May 14, 2009·IEEE Transactions on Nanobioscience·Ken Cham-Fai LeungChristopher H K Cheng

❮ Previous
Next ❯

Related Concepts