Functionalized Asymmetric Bola-Type Amphiphiles for Efficient Gene and Drug Delivery

Nanomaterials
Zheng HuangXiao-Qi Yu

Abstract

The studies of bolaamphiphile-based nanoparticles as delivery vectors are still rudimentary and under development. In this study, several asymmetric bolaamphiphiles containing lysine and another moiety with special functions, such as pH-sensitive or cell-targeting property, were designed and synthesized. The potentials of these bolaamphiphile-based nanoparticles as versatile vectors for both nucleic acids and chemical drugs were studied. With the presence of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), these amphiphiles could be prepared into bolasomes, which showed good DNA binding ability and could condense plasmid DNA into nanoparticles with appropriate size and surface potential.Lys-His, which has a pH-sensitive histidine on one head, exhibited higher transfection efficiency than the symmetric counterpart and comparable efficiency to commercially available transfection reagent. Mechanism studies confirmed that the bolaplexes formed fromLys-Hismight induce the highest cellular uptake and the best endosomal escape ability. On the other hand, these bolaamphiphiles also exhibited good drug loading ability. The self-assembly vesicles could efficiently encapsulate the hydrophobic anti-cancer drug doxorubicin (DOX) in aqu...Continue Reading

References

Sep 26, 1997·Nature·I M Verma, N Somia
Mar 22, 2001·Advanced Drug Delivery Reviews·C W Pouton, L W Seymour
Mar 20, 2003·Bioconjugate Chemistry·Karine FabioPierre Vierling
Apr 10, 2004·Science·Alicia E Smith, Ari Helenius
Jun 10, 2004·Chemical Reviews·Jürgen-Hinrich Fuhrhop, Tianyu Wang
Mar 3, 2005·Journal of the American Chemical Society·Yemin Liu, Theresa M Reineke
Dec 5, 2008·Chemical Reviews·Meredith A Mintzer, Eric E Simanek
Jan 28, 2009·Journal of the American Chemical Society·David A GiljohannChad A Mirkin
Jul 31, 2009·Chemical Communications : Chem Comm·Santanu Bhattacharya, Avinash Bajaj
Oct 12, 2010·Bioconjugate Chemistry·Qiao ZhangSi-Xue Cheng
Oct 16, 2010·Bioconjugate Chemistry·Namrata JainAndrey S Klymchenko
Aug 30, 2011·Accounts of Chemical Research·Xia Guo, Leaf Huang
Jan 3, 2012·International Journal of Pharmaceutics·Namrata JainAndrey S Klymchenko
Aug 14, 2012·Small·Sung Tae KimVincent M Rotello
Nov 28, 2012·Advanced Healthcare Materials·Majad KhanYi-Yan Yang
Oct 8, 2013·Molecular Pharmaceutics·Charles H JonesBlaine A Pfeifer
Dec 18, 2013·Acta Biomaterialia·Wen-Jing YiXiao-Qi Yu
Apr 9, 2014·Biomaterials·Hongmei LiuYiyun Cheng
May 9, 2014·Chemical Communications : Chem Comm·Wen-Jing YiXiao-Qi Yu
Nov 6, 2014·Organic & Biomolecular Chemistry·Zheng HuangXiao-Qi Yu
May 30, 2015·Chemical Communications : Chem Comm·Wen Xiu RenJong Seung Kim
Oct 6, 2015·ACS Central Science·Hanxiang ZengZhibin Guan
Apr 15, 2016·ACS Applied Materials & Interfaces·Chao-Ran LuanXiao-Qi Yu
May 15, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yaqin ZhuZhiyuan Zhong
Aug 27, 2016·Biomacromolecules·Alexander C EldredgeZhibin Guan
Apr 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qian GuoXiao-Qi Yu
Jul 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jingjing SunAmin Cao
Sep 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zheng HuangXiao-Qi Yu

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
protein assay
NMR
dynamic
transmission electron microscopy
silica
column chromatography
ELISA
flow cytometry
Scanning

Software Mentioned

Viewfinder Lite

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.