Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments

Scientific Reports
Qingsu ChengBahram Parvin

Abstract

To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. We also demonstrate that cysteine-functionalized C60-pyrrolidine tris acid can differentiate live and dead microorganisms.

References

Apr 1, 1994·Analytical Biochemistry·D Sehgal, I K Vijay
Dec 6, 2001·Nature·N SanoG A Amaratunga
Nov 5, 2003·Journal of Biochemical and Biophysical Methods·Filip FrederixGuido Maes
Dec 4, 2003·European Journal of Medicinal Chemistry·Susanna BosiMaurizio Prato
May 4, 2004·Methods : a Companion to Methods in Enzymology·Brian DalbyValentina C Ciccarone
Jun 12, 2004·Science·I M Young, J W Crawford
Sep 9, 2005·Organic Letters·David J Maloney, Sidney M Hecht
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P A BottomleyT H Foster
May 4, 2006·Annual Review of Plant Biology·Harsh P BaisJorge M Vivanco
Jul 19, 2008·The Journal of Biological Chemistry·Clelia De-la-PeñaJorge M Vivanco
May 6, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Leshuai W Zhang, Nancy A Monteiro-Riviere
Jun 30, 2009·Physical Chemistry Chemical Physics : PCCP·Haruhisa KatoShinichi Kinugasa
Aug 29, 2009·Nanotechnology·Nadezda NikolićVladimir Trajković
Sep 5, 2009·Nature Protocols·Zhuang LiuHongjie Dai
Feb 2, 2010·European Journal of Medicinal Chemistry·Andrey A ToropovJerzy Leszczynski
May 17, 2012·Mini Reviews in Medicinal Chemistry·M IbrahimA A Elsayed
Jul 25, 2012·Gut Microbes·Torey Looft, Heather K Allen
Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Joel M HymanPaul A Wender
May 3, 2013·PloS One·Peter H Hemmerich, Anna H von Mikecz
Jan 15, 2014·Connective Tissue Research·Qihai LiuLi Jin

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Citations

Nov 11, 2017·Journal of Nanobiotechnology·Qingsu Cheng, Bahram Parvin
Oct 10, 2020·Physical Chemistry Chemical Physics : PCCP·Saunak DasMartin Presselt
Nov 14, 2020·Applied Microbiology and Biotechnology·Qingsu Cheng, Bahram Parvin
Dec 1, 2017·The Journal of Physical Chemistry. a·Johannes FiedlerStefan Y Buhmann
Apr 24, 2018·The Journal of Physical Chemistry. a·Johannes FiedlerStefan Y Buhmann

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Methods Mentioned

BETA
environmental stress
x-ray microtomography
fluorescence microcopy
transmission electron microscopy
confocal microscopy
fluorescence microscopy
chips

Software Mentioned

ImageJ
Promokine

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