Functionalized magnetic nanoparticles attenuate cancer cells proliferation: Transmission electron microscopy analysis

Microscopy Research and Technique
Sultan AkhtarAbdullah Buhaimed

Abstract

The penetration and transportation of nanoparticles (NPs) inside the cancer cells is critical to study. In this article, cancer cells (HCT-116) were treated with functionalized magnetic NPs for the period of 48 hr and studied their ultrastructure by transmission electron microscopy (TEM). The NPs-treated cells were prepared by chemical fixation and sliced into electron-transparent arbitrary sections (200 × 200 μm2 ) by ultramicrotome. Major events of NPs-cell interaction, such as penetration of NPs, encapsulation of NPs into the intracellular compartments, transportation of NPs, and NPs exit, were examined by TEM to understand the mechanism of cell death. The NPs showed the uniform spherical shape with broad size distribution (100-400 nm), while cells displayed irregular morphology with average diameter ~5 μm. Our results showed the successful penetration of NPs deep into the cell, encapsulation, transportation, and exocytosis. Furthermore, we tested the different concentrations (0, 1.5, 12.5, and 50 μg/ml) of NPs on cancer cells and evaluated the cell viability. Laser confocal microscopy and colorimetric analysis together demonstrated that the cell viability is a dose-dependent phenomenon, where 50 μg/ml specimen showed the hi...Continue Reading

References

Jul 1, 1983·Journal of Bacteriology·J DubochetK G Lickfeld
Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·K Richter
Apr 15, 1993·Microscopy Research and Technique·M T Nicolas, J M Bassot
Mar 31, 2005·Journal of Structural Biology·Ashraf Al-AmoudiJacques Dubochet
Oct 18, 2005·Molecular Cancer Therapeutics·David L MorseRobert J Gillies
Apr 13, 2006·Nano Letters·B Devika ChithraniWarren C W Chan
Apr 15, 2006·Mini Reviews in Medicinal Chemistry·K S M Smalley, M Herlyn
Jul 5, 2008·Cancer Cell·Sandra TurcotteAmato J Giaccia
Jul 26, 2008·Nature Nanotechnology·Wen JiangWarren C W Chan
Feb 18, 2009·Bioconjugate Chemistry·Zeljka KrpetićMathias Brust
Sep 29, 2009·Colloids and Surfaces. B, Biointerfaces·Morteza MahmoudiPieter Stroeve
Oct 30, 2009·Chemical Communications : Chem Comm·Erwan FaoucherMathias Brust
Jan 23, 2010·Environmental Science & Technology·Annina GaschenMarianne Geiser
Jan 30, 2010·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Marianne Geiser
Aug 19, 2010·Expert Opinion on Drug Delivery·John W RasmussenDenise G Wingett
Apr 7, 2011·International Journal of Nanomedicine·Weikai ZhangJing Zou
Dec 23, 2011·The Journal of Hand Surgery, European Volume·W BhatS Knight
Jun 19, 2012·BMJ Case Reports·Mohammed Majid AkhtarJaved Akhtar
Jul 31, 2012·Nanoscale·Morteza MahmoudiHojatollah Vali
Sep 25, 2012·Journal of Structural Biology·Stefano RubinoKlaus Leifer
Jul 3, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Christian WeisBen Fabry
Feb 25, 2014·Nature Communications·Hsueh-Cheng ChiangLing-Gang Wu
Jun 19, 2015·Macromolecular Bioscience·Serena MancarellaStefano Leporatti
Sep 4, 2015·Advanced Healthcare Materials·Abhalaxmi SinghSanjeeb K Sahoo
Jan 23, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Rashmirekha PatiAvinash Sonawane

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Citations

Jun 3, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Emily XuAlexandra S Piotrowski-Daspit
Jun 24, 2021·Artificial Cells, Nanomedicine, and Biotechnology·Suhailah S Al-JameelAbdulhadi Baykal

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