Functionalized nanoparticles for liquid atmospheric pressure matrix-assisted laser desorption/ionization peptide analysis

Rapid Communications in Mass Spectrometry : RCM
Kevin TurneyW W Harrison

Abstract

Nanoparticles for the extraction of peptides and subsequent analysis using atmospheric pressure matrix-assisted laser desorption/ionization (APMALDI) have been evaluated. The atmospheric pressure source allows for particles to be directly introduced in the liquid matrix, minimizing sample loss and analysis time. Described in this work are two sample preparation procedures for liquid APMALDI analysis: a C18 functionalized silica nanoparticle for hydrophobic extractions, and an aptamer functionalized magnetite core nanoparticle for rapid, affinity extractions. The C18 particles provide a non-selective support for rapid profiling applications, while the aptamer particles are directed towards reducing the complexity in biological samples. The aptamer functionalized particles provide a more selective analyte-nanoparticle interaction whereby the tertiary structure of the analyte becomes more critical to the extraction. In both cases, the liquid APMALDI matrix provides a support for ionization, and acts as the releasing agent for the analyte-particle interaction. Additionally, analyte enrichment was possible due to the large surface-to-volume ratio of the particles. The experiments conducted with functionalized nanoparticles, in an at...Continue Reading

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Citations

Dec 25, 2010·Parasitology Research·Sampath MarimuthuChinnaperumal Kamaraj
Mar 21, 2007·Journal of the American Society for Mass Spectrometry·Sarah Y ChangC R Cris Wang
Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
Dec 26, 2009·Biosensors & Bioelectronics·Sandrine PerrierEric Peyrin
Jun 25, 2008·Analytica Chimica Acta·Joseph A PhillipsWeihong Tan
Oct 2, 2017·Bioprocess and Biosystems Engineering·Azhar U KhanMohammad Mansoob Khan
Jul 28, 2009·Analytical Chemistry·Josephine RutaEric Peyrin
Sep 14, 2006·Chemical Reviews·Natarajan Venkatesan, Byeang Hyean Kim

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