Functionalized self-assembling peptide hydrogel enhance maintenance of hepatocyte activity in vitro.

Journal of Cellular and Molecular Medicine
Elsa GenoveCarlos E Semino

Abstract

There is a major challenge in maintaining functional hepatocytes in vivo as these cells rapidly lose their metabolic properties in culture. In this work we have developed a bioengineered platform that replaces the use of the collagen I--in the traditional culture sandwich technique--by a defined extracellular matrix analogue, the self-assembling peptide hydrogel RAD16-I functionalized with biologically active motifs. Thus, after examining side by side the two culture systems we have found that in both cases hepatocytes acquired similar parenchymal morphology, presence of functional bile canaliculi structures, CYP3A2 induction by dexamethasone, urea production, secretion of proteins such as apolipoprotein (class A1, E, J), alpha(1)-microglobulin, alpha(1)-macroglobulin, retinol binding protein, fibronectin, alpha(1)-inhibitor III and biotin-dependent carboxylases. Interestingly, by assessing in more detail some other hepatic markers, one of the functionalized matrix analogues--carrying the 67 kD laminin receptor ligand--enhanced the gene expression of albumin, HNF4-alpha, MDR2 and tyrosine aminotransferase. We conclude that the use of a synthetic culture system with designed matrix functionalization has the advantage in controll...Continue Reading

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Citations

Nov 20, 2012·Tissue Engineering. Part a·Núria Marí-BuyéCarlos E Semino
Dec 15, 2010·Journal of Nanobiotechnology·Jonathan WuCarlos E Semino
Aug 1, 2013·Drug Delivery and Translational Research·Veronica A C Puig-Sanvicens, Carlos E Semino
Jul 6, 2014·Advanced Drug Delivery Reviews·Mireia Alemany-Ribes, Carlos E Semino
May 12, 2015·Advanced Drug Delivery Reviews·Kristin M FrenchMichael E Davis
Aug 28, 2012·Advanced Drug Delivery Reviews·Sotirios Koutsopoulos
Mar 27, 2015·Journal of Biomedical Materials Research. Part a·Cristina Martínez-RamosManuel Monleón Pradas
Oct 7, 2011·Journal of Biomedical Materials Research. Part a·Yamato KikkawaMotoyoshi Nomizu
Sep 20, 2011·Acta Biomaterialia·Hongkwan ChoDaria A Narmoneva
Jan 18, 2015·Acta Biomaterialia·Teresa Fernández-MuiñosCarlos E Semino
Mar 5, 2016·Journal of Biomedical Materials Research. Part a·Lourdes Recha-Sancho, Carlos E Semino
Jul 18, 2017·ACS Applied Materials & Interfaces·Honglin JinZhenwei Zou
Jan 1, 2015·Microarrays·Anastasia BachmannAndreas K Nussler
Jan 1, 2014·Hepatology International·Britta BurkhardtAndreas K Nüssler
Nov 20, 2016·Advanced Healthcare Materials·Roman A PerezHae-Won Kim
Aug 2, 2021·Colloids and Surfaces. B, Biointerfaces·M R PoornaUllas Mony
Apr 24, 2015·ACS Applied Materials & Interfaces·Nicole I zur NiedenJulia G Lyubovitsky

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Methods Mentioned

BETA
PCR
ELISA
Protein Assay
electrophoresis
fluorescence microscopy

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