Functionalized surface arrays for spatial targeting of immune cell signaling

Journal of the American Chemical Society
Wageesha SenaratneBarbara Baird

Abstract

The effect of surface topography and chemistry on cellular response is of fundamental importance, especially where living systems encounter device surfaces as in medical implants, tissue engineering, and cell-based sensors. To understand these biological processes on surfaces, there is a widespread interest in tailored surface-active materials produced by a combination of surface chemistry coupled to advanced patterning processes. We utilize self-assembled monolayers (SAMs) as molecular templates with submicrometer-scale spatial resolution to engage and cluster IgE receptors on rat basophilic leukemia (RBL) mast cells. Bioactive templates consisted of gold arrays on silicon with patterns from 1 mum down to 45 nm. These gold arrays served as molecular tethering sites, enabling covalent binding of functionalized self-assembled monolayers of alkanethiols. The free ends of the monolayers were functionalized with 2,4-dinitrophenyl(DNP)-caproate-based ligands which interact specifically with anti-DNP IgE bound to its high affinity cell surface receptor, FcepsilonRI on RBL mast cells. Present results on structures 1 mum down to 600 nm in size indicate that these ligand-immobilized patterned arrays can function as a powerful tool for v...Continue Reading

References

Jan 1, 1996·Annual Review of Biophysics and Biomolecular Structure·D Holowka, B Baird
Jun 17, 1998·Biotechnology Progress·C S ChenD E Ingber
Jun 8, 1999·Annual Review of Immunology·J P Kinet
Jul 12, 2001·Annual Review of Biomedical Engineering·G M WhitesidesD E Ingber
Nov 10, 2001·Annual Review of Biomedical Engineering·A Folch, M Toner
Jun 20, 1996·Chemical Reviews·Abraham Ulman
Apr 8, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Marco ArnoldJoachim P Spatz
Sep 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Min WuBarbara Baird
Nov 19, 2005·Science·Kaspar D MossmanMichael L Dustin
Dec 1, 2005·Journal of the American Chemical Society·Arum Amy YuFrancesco Stellacci

❮ Previous
Next ❯

Citations

Jan 23, 2009·Journal of the American Chemical Society·Karen L ChristmanHeather D Maynard
May 12, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Ethan N ChiangBarbara A Baird
Oct 16, 2013·Nano Letters·Derfogail DelcassianIain E Dunlop
Feb 26, 2009·Chemical Communications : Chem Comm·Wei Luo, Muhammad N Yousaf
Jul 19, 2013·Annual Review of Biomedical Engineering·L C KamM L Dustin
Jan 1, 2009·Materials Research Society Symposia Proceedings·Keyue ShenLance C Kam
Jan 1, 2007·Nanoscale Research Letters·Paula M MendesJon A Preece
May 4, 2010·Biochimica Et Biophysica Acta·Asha Shekaran, Andres J Garcia
Jul 10, 2007·Current Opinion in Immunology·Darrell J IrvineBonnie Huang
Oct 24, 2006·EMBO Reports·Satvajit MayorMiguel A del Pozo
Dec 22, 2007·Chembiochem : a European Journal of Chemical Biology·Kwang-Seuk KoMichael D Burkart
Nov 14, 2008·Angewandte Chemie·Zhen GuYong Chen
Mar 20, 2020·Progress in Materials Science·John R CleggNicholas A Peppas
Mar 24, 2009·Biomacromolecules·K H Aaron LauWolfgang Knoll
Aug 22, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Qin LiWolfgang Knoll
Jul 6, 2014·ACS Applied Materials & Interfaces·Jeong Hun KimKahp-Yang Suh
Dec 1, 2011·Journal of the American Chemical Society·Christopher M KolodziejHeather D Maynard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.