Abstract
A large number of group I introns encode a family of homologous proteins that either promote intron splicing (maturases) or are site-specific DNA endonucleases that function in intron mobility (a process called "homing"). Genetic studies have shown that some of these proteins have both activities, yet how a single protein carries out both functions remains obscure. The similarity between respective DNA-binding sites and the RNA structure near the 5' and 3' splice sites has fueled speculation that such proteins may use analogous interactions to perform both functions. The Aspergillus nidulans mitochondrial COB group I intron encodes a bi-functional protein, I-AniI, that has both RNA maturase and site-specific DNA endonuclease activities in vitro. Here, we show that I-AniI shows distinctive features of the endonuclease family to which it belongs, including highly specific, tight binding and sequential DNA strand cleavage. Competition experiments demonstrate that I-AniI binds the COB intron RNA even in saturating concentrations of its DNA target site substrate, suggesting that the protein has a separate binding site for RNA. In addition, we provide evidence that two different DNA-binding site mutants of I-AniI have little effect o...Continue Reading
References
Nov 1, 1990·Trends in Biochemical Sciences·A M Lambowitz, P S Perlman
Feb 10, 1989·Cell·A M Lambowitz
Aug 12, 1982·Nature·G DujardinP P Slonimski
Feb 1, 1983·Cell·L A HensgensL A Grivell
Sep 1, 1995·Microbiology·M Mikkonen, T Alatossava
Mar 17, 1995·The Journal of Biological Chemistry·F S Gimble, B W Stephens
Dec 13, 1996·Cell·M G CapraraE Westhof
May 16, 1997·Cell·X DuanF A Quiocho
Jul 1, 1997·Nucleic Acids Research·M TurmelC Lemieux
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y HoR B Waring
Feb 12, 1998·Nucleic Acids Research·J Z DalgaardI S Mian
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y ChoJ D Palmer
Sep 16, 1999·Cellular and Molecular Life Sciences : CMLS·M S Jurica, B L Stoddard
Oct 16, 1999·Nucleic Acids Research·K KomoriY Ishino
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M R Goddard, A Burt
Apr 8, 2000·FEMS Microbiology Letters·F S Gimble
Oct 4, 2000·Molecular & General Genetics : MGG·T SzczepanekJ Lazowska
Jan 6, 2001·RNA·S B Rho, S A Martinis
Sep 15, 2001·Nucleic Acids Research·B S Chevalier, B L Stoddard
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gurminder S BassiKevin M Weeks
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christopher A MyersAlan M Lambowitz
May 7, 2002·RNA·Amanda SolemMark G Caprara
Sep 10, 2002·Nature Structural Biology·Carmen M MoureFlorante A Quiocho
Citations
Sep 29, 2004·Nature Structural & Molecular Biology·Nikolai Windbichler, Renée Schroeder
Aug 24, 2005·Nature Structural & Molecular Biology·Antonella LongoKevin M Weeks
Apr 5, 2008·Nucleic Acids Research·Benjamin J KasparMark G Caprara
Dec 24, 2008·Nucleic Acids Research·Ryo TakeuchiBarry L Stoddard
Mar 13, 2012·Nucleic Acids Research·Gregory K Taylor, Barry L Stoddard
Nov 17, 2011·Phytopathology·Y N YinC L Xiao
Nov 25, 2003·Genes & Development·Jill M BolducBarry L Stoddard
Sep 11, 2007·BMC Evolutionary Biology·Peik HaugenKathleen M Pryer
Dec 14, 2006·RNA·Mark G CapraraBenjamin J Kaspar
Mar 17, 2005·RNA·Maureen E DowningMark G Caprara
Mar 5, 2014·Mobile DNA·Barry L Stoddard
Nov 1, 2011·Fungal Biology·Mohamed Hafez, Georg Hausner
Aug 28, 2007·Journal of Molecular Biology·Michelle Scalley-KimBarry L Stoddard
Jun 19, 2008·The Plant Journal : for Cell and Molecular Biology·Andéol Falcon de LongevialleIan Small
Nov 9, 2004·Current Opinion in Genetics & Development·Austin Burt, Vassiliki Koufopanou
Aug 12, 2014·Fungal Biology·Mohamed HafezGeorg Hausner
Dec 7, 2010·Biochimie·Dingding MoYi Zhang
Aug 2, 2006·FEMS Yeast Research·Tomasz SzczepanekPawel Golik
May 12, 2006·Pest Management Science·Valeria GrassoUlrich Gisi
Mar 17, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Aubrey D N J de Grey
Apr 22, 2005·Molecular Biology and Evolution·Vassiliki Koufopanou, Austin Burt
Feb 10, 2017·Nucleic Acids Research·Kyle JacobyAndrew M Scharenberg
Dec 11, 2003·Genes & Development·Marlene Belfort