Functionally orthologous viral and cellular microRNAs studied by a novel dual-fluorescent reporter system.

PloS One
Xiangyu YouXian-En Zhang

Abstract

Recent research raised the possibility that some viral microRNAs (miRNAs) may function as orthologs of cellular miRNAs. In the present work, to study the functional orthologous relationships of viral and cellular miRNAs, we first constructed a dual-fluorescent protein reporter vector system for the easy determination of miRNA function. By expressing the miRNAs and the indicator and internal control fluorescent proteins individually from a single vector, this simple reporter system can be used for miRNA functional assays that include visualizing miRNA activity in live cells. Sequence alignments indicated that the simian virus 40 (SV40) encoded miRNA sv40-mir-S1-5p contains a seed region identical to that of the human miRNA hsa-miR423-5p. Using the new reporter system, it was found that sv40-mir-S1-5p and hsa-miR423-5p downregulate the expression of common artificial target mRNAs and some predicted biological targets of hsa-miR423-5p, demonstrating that they are functional orthologs. The human immunodeficiency virus 1 (HIV-1) encoded hiv1-miR-N367 also contains a seed sequence identical to that of the human miRNA hsa-miR192. Functional assays showed that hiv1-miR-N367 and hsa-miR192 could downregulate common artificial and predic...Continue Reading

References

Dec 1, 1995·Molecular and Cellular Biology·H YangT Lindsten
Oct 27, 2001·Science·M Lagos-QuintanaT Tuschl
Oct 27, 2001·Science·R C Lee, V Ambros
Apr 18, 2002·Genes & Development·Patrick J PaddisonDouglas S Conklin
Aug 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yan ZengBryan R Cullen
Dec 17, 2004·Retrovirology·Shinya OmotoYoichi R Fujii
Feb 22, 2005·The Journal of General Virology·Shinya Omoto, Yoichi R Fujii
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Sep 21, 2007·Journal of Virology·Rebecca L SkalskyRolf Renne
Dec 14, 2007·Nature·Eva GottweinBryan R Cullen
Oct 24, 2008·Journal of Virology·Yuguang ZhaoVenugopal Nair
Apr 29, 2009·The International Journal of Biochemistry & Cell Biology·Yoshio KatoHiroshi Asahara
Jun 7, 2011·Biochimica Et Biophysica Acta·Laurent Houzet, Kuan-Teh Jeang

❮ Previous
Next ❯

Citations

Apr 22, 2014·Current Opinion in Virology·Michael J Imperiale
Aug 30, 2013·Virology Journal·Ole LagatieLieven J Stuyver
Nov 26, 2015·Analytical Cellular Pathology (Amsterdam)·Rehab M SamakaNanes S Holah
Jan 29, 2016·Biomarker Insights·Jennifer LoutenGabrielle Holenstein
Nov 23, 2019·HIV Medicine·J Sadri NahandM R Hamblin
Sep 16, 2020·Non-coding RNA·Alessia GalloPier Giulio Conaldi
Aug 22, 2018·Viruses·Annie Bernier, Selena M Sagan
May 24, 2013·Chemical Reviews·Haifeng DongXueji Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Fluorescence
transfection
fluorescence microscopy
transfections
electrophoresis

Software Mentioned

TARGETSCAN

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.