Functions for rice RFL in vegetative axillary meristem specification and outgrowth

Journal of Experimental Botany
Gauravi M DeshpandeUsha Vijayraghavan

Abstract

Axillary meristems (AMs) are secondary shoot meristems whose outgrowth determines plant architecture. In rice, AMs form tillers, and tillering mutants reveal an interplay between transcription factors and the phytohormones auxin and strigolactone as some factors that underpin this developmental process. Previous studies showed that knockdown of the transcription factor gene RFL reduced tillering and caused a very large decrease in panicle branching. Here, the relationship between RFL, AM initiation, and outgrowth was examined. We show that RFL promotes AM specification through its effects on LAX1 and CUC genes, as their expression was modulated on RFL knockdown, on induction of RFL:GR fusion protein, and by a repressive RFL-EAR fusion protein. Further, we report reduced expression of auxin transporter genes OsPIN1 and OsPIN3 in the culm of RFL knockdown transgenic plants. Additionally, subtle change in the spatial pattern of IR4 DR5:GFP auxin reporter was observed, which hints at compromised auxin transport on RFL knockdown. The relationship between RFL, strigolactone signalling, and bud outgrowth was studied by transcript analyses and by the tillering phenotype of transgenic plants knocked down for both RFL and D3. These data ...Continue Reading

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Citations

Mar 5, 2016·Plant Science : an International Journal of Experimental Plant Biology·Deepak BajajSwarup K Parida
Apr 4, 2017·The Plant Journal : for Cell and Molecular Biology·Juan WangShanguo Yao
Nov 12, 2018·Journal of Integrative Plant Biology·Pan LiXin-Guang Zhu
Feb 13, 2019·Journal of Experimental Botany·Grace L ChongloiUsha Vijayraghavan
Dec 29, 2020·The Plant Journal : for Cell and Molecular Biology·Zhongyuan ChangChengqiang Ding

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