Functions of MiRNA-128 on the regulation of head and neck squamous cell carcinoma growth and apoptosis

PloS One
Belinda HauserXinbin Gu

Abstract

Incidence of head and neck squamous cell carcinoma (HNSCC) has continuously increased in past years while its survival rate has not been significantly improved. There is a critical need to better understand the genetic regulation of HNSCC tumorigenesis and progression. In this study, we comprehensively analyzed the function of miRNA-128 (miR-128) in the regulation of HNSCC growth and its putative targets in vitro and in vivo systems. The function and targets of miR-128 were investigated in human HNSCC cell lines (JHU-13 and JHU-22), which were stably transfected with the miR-128 gene using a lentiviral delivery system. The expression levels of miR-128 and its targeted proteins were analyzed with qRT-PCR, Western blotting and flow cytometry. The binding capacity of miRNA-128 to its putative targets was determined using a luciferase report assay. MTT, colony formation, and a tumor xenograft model further evaluated the effects of miR-128 on HNSCC growth. We generated two miR-128 stably transfected human HNSCC cell lines (JHU-13miR-128 and JHU-22miR-128). Enforced expression of miR-128 was detected in both cultured JHU-13miR-128 and JHU-22miR-128 cell lines, approximately seventeen to twenty folds higher than in vector control cell...Continue Reading

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Citations

Mar 16, 2016·Biochimica Et Biophysica Acta·Filomena de Nigris
Apr 18, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shuxin LiangYong Zhang
Oct 27, 2015·World Journal of Gastroenterology : WJG·Qing YangLei Ding
Nov 11, 2017·Cancer Biomarkers : Section a of Disease Markers·Limei FanFei Wu
Mar 24, 2018·Oncotarget·Herlinda GuzmanIrene Munk Pedersen
Nov 19, 2020·Heliyon·Soudeh Ghafouri-FardZeinab Shirvani Farsani
Feb 13, 2021·Frontiers in Oncology·Xinxin ZhouTeng Ma

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Methods Mentioned

BETA
xenograft
PCR
transfection
protein assay
flow cytometry
xenografts

Software Mentioned

TargetScan
CellQuest Pro
Tar
Pic

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