Functions of OsDof25 in regulation of OsC4PPDK

Plant Molecular Biology
Y ZhangP B F Ouwerkerk

Abstract

Relative little is known about the functions of the so-called Dof zinc factors in plants. Here we report on the analysis of OsDof25 and show a function in regulation of the important C4 photosynthesis gene, OsC4PPDK in rice. Over-expression of OsDof25 enhanced the expression of OsC4PPDK in transient expression experiments by binding in a specific way to a conserved Dof binding site which was confirmed by yeast and in vitro binding studies. Expression studies using promoter GUS plants as well as qPCR experiments showed that OsDof25 expressed in different tissues including both photosynthetic and non-photosynthetic organs and that expression of OsDof25 was partially overlapping with the OsC4PPDK gene. Conclusive evidence for a role of OsDof25 in regulation of C4PPDK came from loss-of-function and gain-of-function experiments with transgenic rice, which showed that down-regulation or over-expression of OsDof25 correlated with OsC4PPDK expression and that OsDof25 has functions as transcriptional activator.

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Citations

Dec 13, 2016·Journal of Experimental Botany·Gregory ReevesJulian M Hibberd
Apr 1, 2018·Functional Plant Biology : FPB·Qing YangTianzhong Li
Dec 9, 2020·Plant Science : an International Journal of Experimental Plant Biology·Jinhe WangMin Li
Sep 30, 2021·The Plant Journal : for Cell and Molecular Biology·Luyao YuMilton Brian Traw

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Datasets Mentioned

BETA
ABF51012
AY061966
AF234312
AF294978
KC996733
KP772258
KC996732

Methods Mentioned

BETA
PCR
transfection
protein assay
electrophoretic mobility shift
one-hybrid
transgenic
electro
transgenics

Software Mentioned

ClustalX
MEG4
MEGA4

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