PMID: 11333026May 3, 2001Paper

Functions of SR proteins in the U12-dependent AT-AC pre-mRNA splicing pathway

RNA
M L Hastings, A R Krainer

Abstract

SR proteins play critical roles in the major pre-mRNA splicing pathway. A second pathway processes U12-dependent AT-AC introns. We demonstrate, by biochemical complementation, the requirement for SR proteins in splicing of AT-AC introns. Whereas SR proteins were sufficient to activate splicing of a P120 AT-AC intron, splicing of a sodium channel AT-AC intron required an additional nuclear fraction. Individual recombinant SR proteins promoted splicing of both substrates, but displayed marked preferences. SR proteins supported basal AT-AC splicing, and also splicing stimulation via a downstream enhancer or conventional 5' splice site. Analysis of chimeric transcripts revealed that information dispersed throughout exons and introns dictates SR protein specificity and the requirement for the additional nuclear fraction. Thus, SR proteins function in both major and minor splicing pathways, and in coordinating the activities of both spliceosomes via exon definition. These results suggest that despite the substantial differences in intron consensus sequences and in four of the five snRNPs in each spliceosome, at least some of the interactions involving SR proteins are conserved between the two pathways.

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P D Zamore, M R Green
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K A Montzka, J A Steitz
Jul 20, 1974·Lancet·P D ExonJ M Malins
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·W Y Tarn, J A Steitz
May 9, 1998·Genes & Development·B J BlencoweP A Sharp
Jul 14, 1998·Molecular Cell·K J Hertel, T Maniatis
Jan 14, 1999·Molecular Cell·C B BurgeP A Sharp
Jan 14, 1999·Genes & Development·M V MurrayA R Krainer
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·K J Hertel, T Maniatis
Nov 5, 1999·Methods in Molecular Biology·A Mayeda, A R Krainer
Nov 5, 1999·Methods in Molecular Biology·A Mayeda, A R Krainer
Nov 5, 1999·Methods in Molecular Biology·A M Zahler
Jan 11, 2000·Molecular and Cellular Biology·H X LiuA R Krainer

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Citations

May 23, 2002·RNA·Timothy S McConnellJoan A Steitz
Jul 3, 2007·Nature Structural & Molecular Biology·Haihong Shen, Michael R Green
Dec 21, 2004·Nature Structural & Molecular Biology·Michelle L HastingsAdrian R Krainer
Jun 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Heli K J PessaMikko J Frilander
Jun 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joan A SteitzRichard A Padgett
Oct 29, 2005·Nucleic Acids Research·Pablo Arrisi MercadoFrancisco E Baralle
Mar 19, 2008·Molecular and Cellular Biology·Janne J TurunenMikko J Frilander
Apr 25, 2002·Molecular and Cellular Biology·Claudia SchneiderReinhard Lührmann
Jun 21, 2007·PloS One·Michelle L HastingsAdrian R Krainer
Oct 6, 2005·Biological Chemistry·Cindy L Will, Reinhard Lührmann
Nov 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·J GlanzerJ Eberwine
Oct 5, 2010·Human Molecular Genetics·Francine M JodelkaMichelle L Hastings
Mar 3, 2011·Neurochemical Research·Joachim KrebsMarek Michalak
Oct 18, 2012·Wiley Interdisciplinary Reviews. RNA·Janne J TurunenMikko J Frilander
Jan 9, 2004·Acta Biologica Hungarica·T Rauch, Ibolya Kiss
Dec 17, 2014·PloS One·Claribel D WeeMichelle L Hastings
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Abhijit A Patel, Joan A Steitz
Jan 22, 2005·Molecular Biology and Evolution·Lesley Collins, David Penny
Dec 9, 2008·The Biochemical Journal·Jennifer C Long, Javier F Caceres
Sep 28, 2001·Nucleic Acids Research·A Levine, R Durbin

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