Functions of the ORF9-to-ORF12 gene cluster in varicella-zoster virus replication and in the pathogenesis of skin infection.

Journal of Virology
Xibing CheAnn M Arvin

Abstract

The gene cluster composed of varicella-zoster virus (VZV) open reading frame 9 (ORF9) to ORF12 encodes four putative tegument proteins and is highly conserved in most alphaherpesviruses. In these experiments, the genes within this cluster were deleted from the VZV parent Oka (POKA) individually or in combination, and the consequences for VZV replication were evaluated with cultured cells in vitro and with human skin xenografts in SCID mice in vivo. As has been reported for ORF10, ORF11 and ORF12 were dispensable for VZV replication in melanoma and human embryonic fibroblast cells. In contrast, deletion of ORF9 was incompatible with the recovery of infectious virus. ORF9 localized to the virion tegument and formed complexes with glycoprotein E, which is an essential protein, in VZV-infected cells. Recombinants lacking ORF10 and ORF11 (POKADelta10/11), ORF11 and ORF12 (POKADelta11/12), or ORF10, ORF11 and ORF12 (POKADelta10/11/12) were viable in cultured cells. Their growth kinetics did not differ from those of POKA, and nucleocapsid formation and virion assembly were not disrupted. In addition, these deletion mutants showed no differences compared to POKA in infectivity levels for primary human tonsil T cells. Deletion of ORF12 ...Continue Reading

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Citations

Jun 14, 2012·Herpesviridae·Paul R KinchingtonRobert L Hendricks
Sep 7, 2014·Virus Research·Ayaka OkadaHideto Fukushi
Feb 11, 2014·Nature Reviews. Microbiology·Leigh ZerboniAnn M Arvin
Oct 16, 2013·International Journal of Cancer. Journal International Du Cancer·Seung-Tae LeeJoseph Wiemels
Aug 27, 2015·Virology Journal·Robyn N HallTimothy J Mahony
Jul 26, 2015·Science China. Life Sciences·Wei WangNingShao Xia
Dec 5, 2014·Journal of Virology·Laura RivaCatherine Sadzot-Delvaux
Sep 17, 2011·Expert Review of Vaccines·Mark QuinlivanD Scott Schmid
Oct 2, 2009·Clinical and Vaccine Immunology : CVI·Stanley A Plotkin
Aug 28, 2020·Frontiers in Microbiology·Liping WuXiaoyue Chen
May 26, 2018·Journal of Virology·Marielle LebrunCatherine Sadzot-Delvaux
Nov 23, 2021·PLoS Pathogens·Shirley E BraspenningWerner J D Ouwendijk

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