Fundamental Ca2+ signaling mechanisms in mouse dendritic cells: CRAC is the major Ca2+ entry pathway

The Journal of Immunology : Official Journal of the American Association of Immunologists
Hsu SfG P Ahern

Abstract

Although Ca(2+)-signaling processes are thought to underlie many dendritic cell (DC) functions, the Ca(2+) entry pathways are unknown. Therefore, we investigated Ca(2+)-signaling in mouse myeloid DC using Ca(2+) imaging and electrophysiological techniques. Neither Ca(2+) currents nor changes in intracellular Ca(2+) were detected following membrane depolarization, ruling out the presence of functional voltage-dependent Ca(2+) channels. ATP, a purinergic receptor ligand, and 1-4 dihydropyridines, previously suggested to activate a plasma membrane Ca(2+) channel in human myeloid DC, both elicited Ca(2+) rises in murine DC. However, in this study these responses were found to be due to mobilization from intracellular stores rather than by Ca(2+) entry. In contrast, Ca(2+) influx was activated by depletion of intracellular Ca(2+) stores with thapsigargin, or inositol trisphosphate. This Ca(2+) influx was enhanced by membrane hyperpolarization, inhibited by SKF 96365, and exhibited a cation permeability similar to the Ca(2+) release-activated Ca(2+) channel (CRAC) found in T lymphocytes. Furthermore, ATP, a putative DC chemotactic and maturation factor, induced a delayed Ca(2+) entry with a voltage dependence similar to CRAC. Moreove...Continue Reading

References

Nov 30, 1992·FEBS Letters·A P NaumovG N Mozhayeva
Feb 14, 1992·Analytical Biochemistry·S P Brooks, K B Storey
Oct 4, 1990·Journal of Immunological Methods·M T CrowleyR M Steinman
Jul 1, 1995·Pflügers Archiv : European journal of physiology·M Hoth
Oct 9, 1995·FEBS Letters·H G Fischer, C Eder
Jan 1, 1995·Annual Review of Immunology·R S Lewis, M D Cahalan
Jun 1, 1993·The Journal of Physiology·M Hoth, R Penner
Apr 1, 1993·Immunological Reviews·E BaixerasC Martínez
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A Zweifach, R S Lewis
Oct 22, 1993·Cell·J W Putney, G S Bird
Jan 1, 1997·Annual Review of Immunology·M BaggioliniB Moser
Jun 1, 1997·Current Opinion in Neurobiology·R A North, E A Barnard
Sep 8, 1997·The Journal of Cell Biology·P E RossM D Cahalan
Nov 14, 1997·Physiological Reviews·A B Parekh, R Penner
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Apr 30, 1998·Immunobiology·E DelgadoA Granelli-Piperno
Jun 11, 1999·Advances in Immunology·D BellJ Banchereau
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M SchnurrA Eigler
Dec 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D FerrariF Di Virgilio

❮ Previous
Next ❯

Citations

Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jun IshikawaToshimitsu Yamada
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paul W WacnikMattias Svensson
Sep 17, 2009·Immunological Reviews·Russell D Salter, Simon C Watkins
Dec 2, 2011·Annals of the New York Academy of Sciences·Stefan Feske
Dec 16, 2011·Experimental & Molecular Medicine·Yi WangChunquan Zheng
Jan 15, 2016·American Journal of Physiology. Cell Physiology·Nicolas Demaurex, Paula Nunes
Dec 21, 2016·Scientific Reports·Thieu X PhanGerard P Ahern
Aug 19, 2007·Nature Reviews. Immunology·Stefan Feske
Feb 11, 2011·American Journal of Physiology. Cell Physiology·Nicole HeiseFlorian Lang
Jun 7, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Benoit DevogelaereHumbert De Smedt
Apr 16, 2011·American Journal of Respiratory Cell and Molecular Biology·Zhifei ShaoDevendra K Agrawal
Feb 4, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ekaterina ShumilinaFlorian Lang
May 24, 2013·PloS One·Romain FélixFlorence Velge-Roussel
Apr 19, 2013·American Journal of Physiology. Cell Physiology·Ekaterina ShumilinaFlorian Lang
Mar 8, 2013·Molecular Biology of the Cell·Banyoon CheonRafael A Fissore
Nov 26, 2015·British Journal of Pharmacology·A ParentiS Benemei
Oct 31, 2015·Scientific Reports·Meerim K NurbaevaRodrigo S Lacruz
Nov 22, 2017·Nature Communications·Sophia MaschalidiBénédicte Manoury
Jan 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mark J MillerIan Parker
Nov 5, 2002·Nature Cell Biology·Kartik VenkatachalamDonald L Gill
Sep 1, 2006·Pflügers Archiv : European journal of physiology·Anant B Parekh
Feb 20, 2002·FEBS Letters·Peta J O'ConnellGerard P Ahern
Jul 15, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Adriana Sumoza-ToledoSantiago Partida-Sánchez
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole MatznerFlorian Lang
May 12, 2015·Journal of Immunology Research·Rodrigo ArreolaLenin Pavón
Mar 30, 2010·The Journal of Biological Chemistry·Mirko VukcevicSusan Treves
Oct 26, 2010·Journal of Leukocyte Biology·Bidhan C BandyopadhyayGerard P Ahern
Jan 23, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jing YanFlorian Lang
Jun 26, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martin VaethStefan Feske
Apr 12, 2015·Annual Review of Immunology·Stefan FeskeEdward Y Skolnik
Nov 28, 2017·Nature Communications·Paula Nunes-HaslerNicolas Demaurex
Feb 27, 2009·Journal of Immunotherapy·Gregory A DekabanPeta J O'Connell
Apr 1, 2011·American Journal of Physiology. Cell Physiology·Ekaterina ShumilinaFlorian Lang
Mar 25, 2005·Physiological Reviews·Anant B Parekh, James W Putney
Apr 5, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Meerim K NurbaevaFlorian Lang
Sep 17, 2009·Immunological Reviews·Michael D Cahalan, K George Chandy
Sep 3, 2013·Therapeutic Advances in Neurological Disorders·Stefan Bittner, Sven G Meuth

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Current Opinion in Immunology
Masatsugu Oh-hora, Anjana Rao
Cellular and Molecular Neurobiology
Zhengchang SuJ E Blalock
The Journal of Immunology : Official Journal of the American Association of Immunologists
Nicole MatznerFlorian Lang
© 2021 Meta ULC. All rights reserved