Fundamental differences in spontaneous synaptic inhibition between deep and superficial layers of the rat entorhinal cortex

Hippocampus
Gavin L WoodhallRoland S G Jones

Abstract

We have previously shown that there are clear differences between spontaneous excitatory synaptic currents recorded in layers V and II of the rat entorhinal cortex (EC) in vitro, and have suggested that these might contribute to a more pronounced susceptibility of the deeper layer to epileptogenesis. In the present study, we have made a detailed comparison of spontaneous synaptic inhibition between the two layers by recording spontaneous inhibitory synaptic currents (sIPSCs) using whole-cell patch-clamp techniques in EC slices. Pharmacological studies indicated that sIPSCs were mediated exclusively by gamma-aminobutyric acid (GABA)(A) receptors. There was little difference in average amplitudes, rise or decay times of sIPSCs in layer II compared with layer V. However, in the former, events occurred at 4-5 times the frequency seen in the latter, and frequencies of </=40 Hz were not uncommon. When activity-independent, miniature IPSCs were isolated in tetrodotoxin (TTX), the frequency in layer V was more than halved, but in layer II only a small reduction was seen, and the frequency remained very high. In terms of kinetics, while averaged sIPSCs in each layer were very similar, detailed comparison of individual sIPSCs within laye...Continue Reading

References

Jan 1, 1991·Progress in Neurobiology·W Van der Kloot
Jan 1, 1990·Acta Neuropathologica·J H KimD D Spencer
Sep 1, 1988·Epilepsy Research·W A WilsonD V Lewis
Sep 23, 1987·Neuroscience Letters·F Lotstra, J J Vanderhaeghen
Feb 1, 1995·Journal of Neurophysiology·M AvoliI Kurcewicz
Mar 16, 1995·Brain Research. Developmental Brain Research·S N Hoffman, D A Prince
Oct 24, 1994·Brain Research·E H Bertram, J F Cornett
Jul 1, 1994·Epilepsia·S S Spencer, D D Spencer
Nov 1, 1993·The Journal of Comparative Neurology·E SorianoM Frotscher
Aug 14, 1996·JAMA : the Journal of the American Medical Association·M R SperlingC Plummer
Apr 1, 1996·Journal of Neurophysiology·P A Salin, D A Prince
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·Z NusserP Somogyi
Dec 2, 1996·The Journal of Comparative Neurology·A MartínezM Frotscher

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Citations

Feb 2, 2008·The Journal of Physiology·Philip de GuzmanMassimo Avoli
Dec 25, 2015·Neurobiology of Disease·Rüdiger KöhlingMassimo Avoli
Aug 9, 2008·Molecular and Cellular Neurosciences·Pan-Yue Deng, Saobo Lei
Dec 13, 2006·The Journal of Comparative Neurology·James J Chrobak, David G Amaral
Aug 7, 2007·IEEE Transactions on Information Technology in Biomedicine : a Publication of the IEEE Engineering in Medicine and Biology Society·Etienne LabytFabrice Wendling
Jul 21, 2009·Neuropharmacology·Nicola H MorganGavin L Woodhall
Jul 14, 2021·Physiological Reviews·John J TukkerDietmar Schmitz

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