Fundamentally distinct roles of thyroid hormone receptor isoforms in a thyrotroph cell line are due to differential DNA binding.

Molecular Endocrinology
Maria I ChiamoleraFredric E Wondisford

Abstract

Thyroid hormones have a profound influence on human development and disease. The hypothalamic-pituitary-thyroid axis involves finely tuned feedback mechanisms to maintain thyroid hormone (TH) levels. Despite the important role of TH-negative feedback in regulating this axis, the mechanism by which this occurs is not clearly defined. Previous in vivo studies suggest separate roles for the two thyroid hormone receptor isoforms, THRA and THRB, in this axis. We performed studies using a unique pituitary thyrotroph cell line (TαT1.1) to determine the relative roles of THRA and THRB in the regulation of Tshb. Using chromatin immunoprecipitation assays, we found that THRB, not THRA, bound to the Tshb promoter. By selectively depleting THRB, THRA, or both THRA and THRB in TαT1.1 cells, we found that simultaneous knockdown of both THRB and THRA abolished T(3)-mediated down-regulation of Tshb at concentrations as high as 100 nm T(3). In contrast, THRA knockdown alone had no effect on T(3)-negative regulation, whereas THRB knockdown alone abolished T(3)-mediated down-regulation of Tshb mRNA levels at 10 nm but not 100 nm T(3) concentrations. Interestingly, chromatin immunoprecipitation assays showed that THRA becomes enriched on the Tshb ...Continue Reading

References

Jan 1, 1989·Endocrine Research·M A ShupnikE C Ridgway
Nov 1, 1989·Endocrine Reviews·M A ShupnikW W Chin
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K J KollerR T Zoeller
Jun 9, 1995·The Journal of Biological Chemistry·O CohenF E Wondisford
Jan 23, 1999·The Journal of Clinical Investigation·E D AbelF E Wondisford
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·P E MacchiaS Refetoff
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 4, 2002·Methods in Molecular Biology·Karine GauthierJacques Samarut
Nov 7, 2002·The Journal of Biological Chemistry·Nobuyuki ShibusawaFredric E Wondisford
Aug 20, 2003·The Journal of Clinical Investigation·Nobuyuki ShibusawaFredric E Wondisford
Aug 22, 2003·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Fredric E Wondisford
Dec 13, 2005·The Journal of Biological Chemistry·Amisra A NikrodhanondFredric E Wondisford
Dec 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Anders BerkenstamJohn D Baxter
Apr 23, 2009·Endocrinology·Kristen R Vella, Anthony N Hollenberg
May 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Danielle S MachadoFredric E Wondisford
Aug 29, 2009·The Journal of Endocrinology·Beatriz S AmorimMiriam O Ribeiro
Jan 7, 2010·Endocrine Reviews·Sheue-Yann ChengPaul J Davis
Feb 23, 2011·Endocrinology·Miriam O Ribeiro, Antonio C Bianco
Apr 5, 2011·Molecular and Cellular Endocrinology·Jennifer S JanssenBryan R Haugen
Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Laura FozzattiSheue-yann Cheng
Dec 16, 2011·The New England Journal of Medicine·Elena BochukovaKrishna Chatterjee

❮ Previous
Next ❯

Citations

Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Fabrice ChatonnetFrederic Flamant
Jan 10, 2013·PloS One·Jean Z LinPaul Webb
Jan 16, 2013·Endocrine-related Cancer·Lars C Moeller, Dagmar Führer
Sep 5, 2013·Thyroid : Official Journal of the American Thyroid Association·Antonio C BiancoUNKNOWN American Thyroid Association Task Force on Approaches and Strategies to Investigate Thyroid Hormone Economy and Action
Aug 20, 2014·Nature Reviews. Endocrinology·Tânia M Ortiga-CarvalhoFredric E Wondisford
Dec 5, 2015·Frontiers in Endocrinology·Rudolf HoermannJohannes W Dietrich
Oct 11, 2015·Cell Calcium·Melanija TomićStanko S Stojilkovic
Apr 12, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Alexander G Little
Apr 10, 2015·Journal of Neuroendocrinology·R S DezonneF C Gomes
Jun 23, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Brenda J Mengeling, J David Furlow
Mar 5, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·AlberTinka J MurkArno C Gutleb
Jan 8, 2015·The Journal of Endocrinology·Patricia Joseph-BravoJean-Louis Charli
Apr 27, 2016·Heart Failure Reviews·Alice AccorroniRiccardo Zucchi
Jul 26, 2016·Frontiers in Endocrinology·Frédéric Flamant
Aug 16, 2016·Reviews in Endocrine & Metabolic Disorders·Patricia Joseph-BravoJean-Louis Charli
Sep 28, 2017·Journal of Molecular Endocrinology·Paula Bargi-SouzaM T Nunes
Mar 13, 2014·Molecular Endocrinology·Frédéric PicouFrédéric Flamant
May 6, 2014·The Journal of Biological Chemistry·Irene O AninyeFredric E Wondisford
Jul 12, 2019·Frontiers in Endocrinology·Adair Rodríguez-RodríguezJean-Louis Charli
Mar 4, 2020·Thyroid : Official Journal of the American Thyroid Association·Svetlana MinakhinaFredric E Wondisford
Dec 11, 2017·Best Practice & Research. Clinical Endocrinology & Metabolism·Keisuke Ikegami, Takashi Yoshimura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.