Fungal Diversity in Field Mold-Damaged Soybean Fruits and Pathogenicity Identification Based on High-Throughput rDNA Sequencing

Frontiers in Microbiology
Jiang LiuWen-Yu Yang

Abstract

Continuous rain and an abnormally wet climate during harvest can easily lead to soybean plants being damaged by field mold (FM), which can reduce seed yield and quality. However, to date, the underlying pathogen and its resistance mechanism have remained unclear. The objective of the present study was to investigate the fungal diversity of various soybean varieties and to identify and confirm the FM pathogenic fungi. A total of 62,382 fungal ITS1 sequences clustered into 164 operational taxonomic units (OTUs) with 97% sequence similarity; 69 taxa were recovered from the samples by internal transcribed spacer (ITS) region sequencing. The fungal community compositions differed among the tested soybeans, with 42 OTUs being amplified from all varieties. The quadratic relationships between fungal diversity and organ-specific mildew indexes were analyzed, confirming that mildew on soybean pods can mitigate FM damage to the seeds. In addition, four potentially pathogenic fungi were isolated from FM-damaged soybean fruits; morphological and molecular identification confirmed these fungi as Aspergillus flavus, A. niger, Fusarium moniliforme, and Penicillium chrysogenum. Further re-inoculation experiments demonstrated that F. moniliforme...Continue Reading

References

May 1, 2010·Plant & Cell Physiology·Juan J Gutierrez-GonzalezDavid A Sleper
Nov 22, 2011·Journal of Agricultural and Food Chemistry·Pratyusha ChennupatiWucheng Liu
Feb 22, 2013·The ISME Journal·Elie JamiItzhak Mizrahi
Dec 10, 2016·Journal of Agricultural and Food Chemistry·Jiang LiuWenyu Yang

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Methods Mentioned

BETA
PCR
PCA

Software Mentioned

Excel
USEARCH
R package
Mothur
SPSS
BLAST

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