Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings

BioMed Research International
Palanisamy ManikandanVenkatapathy Narendran

Abstract

Fungal aetiology of keratitis/corneal ulcer is considered to be one of the leading causes of ocular morbidity, particularly in developing countries including India. More importantly, Fusarium and Aspergillus are reported commonly implicating corneal ulcer and against this background the present work was undertaken so as to understand the current epidemiological trend of the two fungal keratitis. During the project period, a total of 500 corneal scrapings were collected from suspected mycotic keratitis patients, of which 411 (82.2%) were culture positive for bacteria, fungi, and parasites. Among fungal aetiologies, Fusarium (216, 52.5% of 411) and Aspergillus (68, 16.5% of 411) were predominantly determined. While the study revealed a male preponderance with both the fungal keratitis , it further brought out that polyene compounds (natamycin and amphotericin B) and azoles were active, respectively, against Fusarium spp. and Aspergillus spp. Additionally, 94.1% of culture proven Fusarium keratitis and, respectively, 100% and 63.6% of A. flavus and A. fumigatus were confirmed by multiplex PCR. The sensitivity of the PCR employed in the present study was noted to be 10 fg/μl, 1 pg/μl, and 300 pg/μl of DNA, respectively, for Fusariu...Continue Reading

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Citations

May 14, 2020·Journal of Investigative Medicine High Impact Case Reports·Que Anh TramLe Tran-Anh
Jul 1, 2020·Advanced Materials·Rezvan JamaledinPooyan Makvandi
Sep 26, 2020·Indian Journal of Ophthalmology·Ranjana RohillaBalram Ji Omar
Aug 29, 2020·Translational Vision Science & Technology·Ning HungChing-Hsi Hsiao
Jul 28, 2019·International Journal of Molecular Sciences·Ming-Tse KuoHuey-Ling You
Jan 8, 2021·International Journal of Surgery Case Reports·Lojain AlBathi, Rawan AlShabeeb
Oct 19, 2019·Microbial Pathogenesis·Lingzhi NiuYajuan Zheng
Jan 20, 2021·Journal of Applied Microbiology·M RaiM Alves
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Feb 22, 2021·Indian Journal of Medical Microbiology·Rameshkumar GunasekaranLalitha Prajna
May 1, 2021·Journal of Fungi·Jeremy J HoffmanAstrid Leck

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Methods Mentioned

BETA
scraping
scrapings
PCR
electrophoresis

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Aspergillosis (ASM)

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Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.