Fungicide activity through activation of a fungal signalling pathway

Molecular Microbiology
Kaihei KojimaTetsuro Okuno

Abstract

Fungicides generally inhibit enzymatic reactions involved in fungal cellular biosynthesis. Here we report, for the first time, an example of fungicidal effects through hyperactivation of a fungal signal transduction pathway. The OSC1 gene, encoding a MAP kinase (MAPK) related to yeast Hog1, was isolated from the fungal pathogen Colletotrichum lagenarium that causes cucumber anthracnose. The osc1 knockout mutants were sensitive to high osmotic stress and showed increased resistance to the fungicide fludioxonil, indicating that Osc1 is involved in responses to hyperosmotic stress and sensitivity to fludioxonil. The Osc1 MAPK is phosphorylated under high osmotic conditions, indicating activation of Osc1 by high osmotic stress. Importantly, fludioxonil treatment also activates phosphorylation of Osc1, suggesting that improper activation of Osc1 by fludioxonil has negative effects on fungal growth. In the presence of fludioxonil, the wild-type fungus was not able to infect the host plant because of a failure of appressorium-mediated penetration, whereas osc1 mutants successfully infected plants. Analysis using a OSC1-GFP fusion gene indicated that Osc1 is rapidly translocated to the nucleus in appressorial cells after the addition o...Continue Reading

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J HowardN P Money
Mar 19, 1993·Science·J L BrewsterM C Gustin
Oct 1, 1996·Trends in Microbiology·C von Eichel-StreiberM Thelestam
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·W W MetcalfR S Wolfe
Apr 8, 2000·Molecular Plant-microbe Interactions : MPMI·Y TakanoI Furusawa
Mar 29, 2001·Fungal Genetics and Biology : FG & B·J R Xu
Nov 1, 2001·Fungal Genetics and Biology : FG & B·Y TakanoT Okuno
Feb 19, 2002·Fungal Genetics and Biology : FG & B·Tamara K MillerClaude P Selitrennikoff
Mar 29, 2002·Molecular Microbiology·Kap-Hoon Han, Rolf A Prade
Nov 27, 2002·Bioscience, Biotechnology, and Biochemistry·Noriyuki OchiaiIsamu Yamaguchi
Mar 26, 2003·Annual Review of Phytopathology·Nancy LeeJames W Kronstad
May 1, 2003·Molecular Plant Pathology·Ian E WheelerSarah J Gurr

❮ Previous
Next ❯

Citations

Jun 16, 2011·ACS Chemical Biology·Sandra TückmantelPeter H Schreier
Dec 16, 2006·Nature Reviews. Microbiology·Yong-Sun BahnMaria E Cardenas
Aug 23, 2005·Medical Mycology·G S MayM C Gustin
May 18, 2012·Molecular Plant-microbe Interactions : MPMI·Nguyen Van ThuatJörg Bormann
Sep 1, 2006·Molecular Plant-microbe Interactions : MPMI·Muriel ViaudLaurent Legendre
Nov 1, 2006·Molecular Plant-microbe Interactions : MPMI·Rahim MehrabiGert H J Kema
Jul 13, 2011·Antimicrobial Agents and Chemotherapy·Anke Burger-KentischerSteffen Rupp
Aug 24, 2007·Eukaryotic Cell·Xinhua ZhaoJin-Rong Xu
Dec 7, 2010·Eukaryotic Cell·Jan S Fassler, Ann H West
Jun 2, 2011·Annals of Clinical Microbiology and Antimicrobials·Jong H KimBruce C Campbell
Dec 4, 2012·International Journal of Molecular Sciences·Jong H KimBruce C Campbell
Jul 10, 2007·Bioscience, Biotechnology, and Biochemistry·Kentaro FurukawaKeietsu Abe
Feb 15, 2014·Cellular and Molecular Life Sciences : CMLS·Brigitte M E HayesMark R Bleackley
Nov 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael W VitaliniDeborah Bell-Pedersen
Nov 15, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jong H Kim, Kathleen L Chan
Sep 3, 2008·International Journal of Food Microbiology·Anastasios N MarkoglouBasil N Ziogas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.