Fur Represses Vibrio cholerae Biofilm Formation via Direct Regulation of vieSAB , cdgD , vpsU , and vpsA-K Transcription

Frontiers in Microbiology
He GaoBiao Kan

Abstract

Attached Vibrio cholerae biofilms are essential for environmental persistence and infectivity. The vps loci (vpsU, vpsA-K, and vpsL-Q) are required for mature biofilm formation and are responsible for the synthesis of exopolysaccharide. Transcription of vps genes is activated by the signaling molecule bis-(3'-5')-cyclic di-GMP (c-di-GMP), whose metabolism is controlled by the proteins containing the GGDEF and/or EAL domains. The ferric uptake regulator (Fur) plays key roles in the transcription of many genes involved in iron metabolism and non-iron functions. However, roles for Fur in Vibrio biofilm production have not been documented. In this study, phenotypic assays demonstrated that Fur, independent of iron, decreases in vivo c-di-GMP levels and inhibits in vitro biofilm formation by Vibrio cholerae. The Fur box-like sequences were detected within the promoter-proximal DNA regions of vpsU, vpsA-K, vieSAB, and cdgD, suggesting that transcription of these genes may be under the direct control of Fur. Indeed, the results of luminescence, quantitative PCR (qPCR), electrophoretic mobility shift assay (EMSA), and DNase I footprinting assays demonstrated Fur to bind to the promoter-proximal DNA regions of vpsU, vpsA-K, and cdgD to ...Continue Reading

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Citations

Jun 25, 2021·Frontiers in Microbiology·Yiquan ZhangDongsheng Zhou

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Methods Mentioned

BETA
PBAD
enzyme-linked immunosorbent assay
electrophoresis
PCR
footprinting
Electrophoretic mobility shift assay
ChIP-seq

Software Mentioned

GeneMarker
Sequence Scanner

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