Furfural-tolerant Zymomonas mobilis derived from error-prone PCR-based whole genome shuffling and their tolerant mechanism

Applied Microbiology and Biotechnology
Suzhen HuangMinhua Zhang

Abstract

Furfural-tolerant strain is essential for the fermentative production of biofuels or chemicals from lignocellulosic biomass. In this study, Zymomonas mobilis CP4 was for the first time subjected to error-prone PCR-based whole genome shuffling, and the resulting mutants F211 and F27 that could tolerate 3 g/L furfural were obtained. The mutant F211 under various furfural stress conditions could rapidly grow when the furfural concentration reduced to 1 g/L. Meanwhile, the two mutants also showed higher tolerance to high concentration of glucose than the control strain CP4. Genome resequencing revealed that the F211 and F27 had 12 and 13 single-nucleotide polymorphisms. The activity assay demonstrated that the activity of NADH-dependent furfural reductase in mutant F211 and CP4 was all increased under furfural stress, and the activity peaked earlier in mutant than in control. Also, furfural level in the culture of F211 was also more rapidly decreased. These indicate that the increase in furfural tolerance of the mutants may be resulted from the enhanced NADH-dependent furfural reductase activity during early log phase, which could lead to an accelerated furfural detoxification process in mutants. In all, we obtained Z. mobilis muta...Continue Reading

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Citations

Jan 1, 2020·Biotechnology for Biofuels·Weiting WangMingxiong He
Oct 26, 2018·Biotechnology for Biofuels·Damien Biot-PelletierVincent J J Martin
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Jul 14, 2020·World Journal of Microbiology & Biotechnology·Tatsaporn TodhanakasemSaw Simeon

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Datasets Mentioned

BETA
PRJNA397315

Methods Mentioned

BETA
PCR

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